Wirth T, Glöggler K, Baumruker T, Schmidt M, Horak I
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3327-30. doi: 10.1073/pnas.80.11.3327.
Screening of a 129/J mouse genomic library under nonstringent hybridization conditions with a xenotropic virus-like long terminal repeat (LTR) probe revealed a family of sequences resembling insertion elements (IS) with structural features of solitary retroviral LTRs; these are called LTR-IS. They are interspersed among variable flanking regions of mouse DNA and lack any viral structural genes. LTR-IS elements start and end with 11-base-pair inverted repeats and contain signals implicated in RNA polymerase II transcriptional regulation: C-C-A-A-T, T-A-T-A-A-A, and A-A-T-A-A-A. The members of the family are homologous, but not identical, approximately equal to 500-base-pair-long elements with 4-base-pair target-site duplications on both sites of the element. There are 500 LTR-IS per mouse haploid genome.
在非严格杂交条件下，用嗜异性病毒样长末端重复序列（LTR）探针筛选129/J小鼠基因组文库，发现了一个类似于插入元件（IS）的序列家族，其具有单独逆转录病毒LTR的结构特征；这些被称为LTR-IS。它们散布在小鼠DNA的可变侧翼区域之间，并且缺乏任何病毒结构基因。LTR-IS元件以11个碱基对的反向重复序列开始和结束，并包含与RNA聚合酶II转录调控有关的信号：C-C-A-A-T、T-A-T-A-A-A和A-A-T-A-A-A。该家族成员具有同源性，但并不相同，是长度约为500个碱基对的元件，在元件两侧有4个碱基对的靶位点重复。每个小鼠单倍体基因组中有500个LTR-IS。
