Preston C M, Cordingley M G
J Virol. 1982 Aug;43(2):386-94. doi: 10.1128/JVI.43.2.386-394.1982.
Microinjection of herpes simplex virus (HSV)-infected cell mRNA into Xenopus laevis oocytes resulted in the production of a new exonuclease activity. This enzyme strongly resembled the HSV alkaline exonuclease in many biochemical properties, and hybrid-arrested translation studies showed that it was virus coded, mapping at 0.080 to 0.185 genome map units. Exonuclease mRNA had a size and genome location equivalent to the mRNA encoding V185 in reticulocyte lysates, suggesting that V185 is the exonuclease. The enzyme synthesized in oocytes was found to act as an exonuclease in vivo. Two plasmids containing HSV DNA fragments directed the synthesis of exonuclease when microinjected into oocyte nuclei, and this finding enabled the coding and control sequences for this gene to be localized to 0.155 to 0.185 genome map units.
将单纯疱疹病毒（HSV）感染细胞的mRNA显微注射到非洲爪蟾卵母细胞中，导致产生了一种新的核酸外切酶活性。这种酶在许多生化特性上与HSV碱性核酸外切酶非常相似，杂交阻断翻译研究表明它是病毒编码的，位于基因组图谱单位0.080至0.185处。核酸外切酶mRNA的大小和基因组位置与网织红细胞裂解物中编码V185的mRNA相当，这表明V185就是核酸外切酶。发现在卵母细胞中合成的这种酶在体内作为核酸外切酶起作用。当将两个含有HSV DNA片段的质粒显微注射到卵母细胞核中时，它们指导了核酸外切酶的合成，这一发现使得该基因的编码和控制序列能够定位到基因组图谱单位0.155至0.185处。
