McKnight S L, Gavis E R, Kingsbury R, Axel R
Cell. 1981 Aug;25(2):385-98. doi: 10.1016/0092-8674(81)90057-x.
We have identified a transcriptional control region of the herpes simplex virus tk gene. This finding results from in vivo transcription assays of specific deletion mutants constructed in vitro. A region located between 40 and 100 nucleotides upstream from the putative transcription start site of the tk gene can promote transcription by RNA polymerase form II in the complete absence of the mRNA-coding component of the gene. When the region is deleted enzymatically from a 5' direction, accurate transcriptional expression is reduced by a factor of 50. The small remaining level of accurate transcription is eliminated by deletion of sequences from 32 to 16 nucleotides upstream from the the structural gene. It appears that the sequences between 16 and 32 nucleotides upstream from the 5' terminus of the tk gene are required to specify the exact start site of transcription. Control of both the efficiency of transcription and rough specification of the position of initiation, however, depends on sequences 40--100 nucleotides upstream from the tk structural gene.
我们已经鉴定出单纯疱疹病毒胸苷激酶（tk）基因的一个转录控制区。这一发现源自对体外构建的特定缺失突变体进行的体内转录分析。位于tk基因推定转录起始位点上游40至100个核苷酸之间的区域，在完全没有该基因的mRNA编码成分的情况下，能够促进RNA聚合酶II的转录。当从5'方向酶切删除该区域时，准确的转录表达降低了50倍。通过删除结构基因上游32至16个核苷酸的序列，消除了剩余的少量准确转录。看来，tk基因5'末端上游16至32个核苷酸之间的序列是确定转录精确起始位点所必需的。然而，转录效率的控制以及起始位置的大致确定，取决于tk结构基因上游40 - 100个核苷酸的序列。
