编码21000道尔顿大小类别的玉米醇溶蛋白的玉米基因的分离与特性分析。

Pintor-Toro J A, Langridge P, Feix G

Nucleic Acids Res. 1982 Jul 10;10(13):3845-60. doi: 10.1093/nar/10.13.3845.

Cloning in lambda gt WES phage of EcoRI fragments from maize seedlings DNA led to the isolation of four fragments containing genes coding for 21000 dalton zein proteins. The zein genes, identified by electron microscopic analysis, do not contain intervening sequences detectable by this method. The flanking sequences were analyzed by restriction sites mapping and hybridization and showed areas of homology between each other and with sequences surrounding a previously isolated gene of the 19000 dalton zein class.

从玉米幼苗DNA的EcoRI片段中克隆到λgt WES噬菌体中，导致分离出四个含有编码21000道尔顿玉米醇溶蛋白基因的片段。通过电子显微镜分析鉴定的玉米醇溶蛋白基因，不包含用这种方法可检测到的间隔序列。通过限制性酶切位点图谱分析和杂交对侧翼序列进行了分析，结果显示它们彼此之间以及与先前分离的19000道尔顿玉米醇溶蛋白类基因周围的序列存在同源区域。