一种植物储存蛋白的一级结构：玉米醇溶蛋白。
The primary structure of a plant storage protein: zein.
作者信息
Geraghty D, Peifer M A, Rubenstein I, Messing J
出版信息
Nucleic Acids Res. 1981 Oct 10;9(19):5163-74. doi: 10.1093/nar/9.19.5163.
Abstract
The protein sequence of a representative of the zeins, the major storage proteins of maize, has been derived from the nucleotide sequence of a zein cDNA clone. This cDNA was sequence both by the Maxam and Gilbert and the M13-dideoxy techniques. The nucleotide sequence encompasses the non-translated 3' terminus of the mRNA, the entire coding sequence specifying both the mature zein protein and a small signal peptide, and a portion of the non-translated 5' region. The deduced amino acid composition and the amino-terminal amino acid sequence closely resemble those derived from chemical analysis of the zein protein fraction. The data presented represent the first complete amino acid sequence of a plant storage protein.
摘要
玉米醇溶蛋白是玉米主要的贮藏蛋白，其一种代表性蛋白的序列已从玉米醇溶蛋白cDNA克隆的核苷酸序列推导得出。该cDNA通过马克萨姆-吉尔伯特法和M13双脱氧技术进行了测序。核苷酸序列包括mRNA的非翻译3'末端、指定成熟玉米醇溶蛋白和一个小信号肽的完整编码序列，以及非翻译5'区域的一部分。推导的氨基酸组成和氨基末端氨基酸序列与从玉米醇溶蛋白部分的化学分析得出的结果非常相似。所呈现的数据代表了植物贮藏蛋白的首个完整氨基酸序列。
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