Rosenbusch R F, Reed D E
Vet Microbiol. 1982 May;7(2):109-16. doi: 10.1016/0378-1135(82)90022-0.
The cytopathology and length of latency in single-step growth curves of two isolates of stomatitis papulosa virus are compared in this report. Isolate 721 was obtained from a calf with oral ulcers and isolate 8665 was obtained from a calf with respiratory disease and oral ulcers. In single-step growth curves, the latency period of isolate 721 was 8 h while that of isolate 8665 was 6 h. The cytopathic effect produced by isolate 721 in bovine lung cells was characterized by enlargement of the cell, cell-to-cell adherence and large intracytoplasmic accumulations of viral inclusion material. Isolate 8665 caused rapid cell degeneration and detachment, with small accumulation of viral inclusion material. Neither of the two strains grew in bovine alveolar macrophage cultures or in the respiratory epithelium of fetal bovine tracheal explants. Intragingival inoculation of these isolates in cattle resulted in oral lesions without clinical signs of respiratory of systemic involvement. Virus was recovered from the oral lesions and from nasal secretions for as long as 10 days. Inoculation of dexamethasone-treated cattle resulted in a similar clinical condition although virus was recovered for 20 days from oral lesions and nasal secretions. Seroconversions from negative to 1 : 2560 were detected in inoculated cattle by indirect immunofluorescence.
本报告比较了两种口炎丘疹病毒分离株单步生长曲线中的细胞病理学和潜伏期。分离株721取自一头患有口腔溃疡的小牛，分离株8665取自一头患有呼吸道疾病和口腔溃疡的小牛。在单步生长曲线中，分离株721的潜伏期为8小时，而分离株8665的潜伏期为6小时。分离株721在牛肺细胞中产生的细胞病变效应的特征是细胞肿大、细胞间黏附以及大量病毒包涵体物质在胞质内积聚。分离株8665导致细胞迅速变性和脱落，病毒包涵体物质积聚较少。这两种毒株在牛肺泡巨噬细胞培养物或胎牛气管外植体的呼吸道上皮中均未生长。将这些分离株经龈内接种到牛体内，导致口腔病变，但无呼吸道或全身受累的临床症状。在长达10天的时间里，可从口腔病变和鼻分泌物中分离到病毒。对接种了地塞米松的牛进行接种，尽管在长达20天的时间里可从口腔病变和鼻分泌物中分离到病毒，但临床症状相似。通过间接免疫荧光法在接种的牛中检测到血清转化，从阴性转为1:2560。
