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[革兰氏阴性微生物完整细胞的红外光谱性质]

[Nature of the IR spectra of intact cells of Gram-negative microorganisms].

作者信息

Zhurko N P, Gureeva A A, Rostovtseva N A, Smirnov S G

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1982 Jun(6):47-50.

PMID:6287766
Abstract

The character of changes in the infrared spectra of E. coli in the process of their mechanical disintegration has been studied. The destruction of E. coli cell structures has been shown to produce no changes in the optical density of the main analytical absorption bands in infrared spectra. This fact suggests that the infrared absorption spectra of E. coli are the sum of the spectra of all chemical components of the cell, which is confirmed by the infrared spectral study of E. coli cell-wall preparations. Similar results have been obtained in the study of the preparations of B. pertussis cell walls, protoplasts and intact cells.

摘要

研究了大肠杆菌在机械破碎过程中红外光谱的变化特征。结果表明,大肠杆菌细胞结构的破坏并未导致红外光谱中主要分析吸收带的光密度发生变化。这一事实表明,大肠杆菌的红外吸收光谱是细胞所有化学成分光谱的总和,这一点已通过对大肠杆菌细胞壁制剂的红外光谱研究得到证实。在对百日咳博德特氏菌细胞壁制剂、原生质体和完整细胞的研究中也获得了类似的结果。

相似文献

1
[Nature of the IR spectra of intact cells of Gram-negative microorganisms].[革兰氏阴性微生物完整细胞的红外光谱性质]
Zh Mikrobiol Epidemiol Immunobiol. 1982 Jun(6):47-50.
2
[Comparative study of lipid A of the pertussis microbe O-antigens differing in their toxicity. II. Infrared spectroscopy study of the structure of preparations of B. pertussis lipid A].百日咳微生物O抗原中脂多糖A的毒性差异比较研究。II. 百日咳杆菌脂多糖A制剂结构的红外光谱研究
Zh Mikrobiol Epidemiol Immunobiol. 1978 Aug(8):138-41.
3
Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.大肠杆菌细胞壁和细胞质膜的蛋白质组成。
J Bacteriol. 1970 Nov;104(2):890-901. doi: 10.1128/jb.104.2.890-901.1970.
4
Immunodiffusion analysis of subcellular fractions of Escherichia coli.
Ann Med Exp Biol Fenn. 1966;44(2):216-20.
5
[Bordetella pertussis spheroplasts. 3. Isolation and chemical analysis of spheroplast cell walls].[百日咳博德特氏菌原生质球。3. 原生质球细胞壁的分离与化学分析]
Zentralbl Bakteriol Orig A. 1972 Feb;219(2):204-10.
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[Use of infrared spectroscopy for studying the chemical composition of yeast cell walls].[利用红外光谱法研究酵母细胞壁的化学成分]
Mikrobiologiia. 1977 Jul-Aug;46(4):667-71.
7
[Isolation of various structural elements of gram-negative bacteria of the Bordetella genus and a study of their properties. II. Isolation of the cytoplasmic membrane fraction from the protoplasts of B. pertussis and a study of their chemical and enzymatic properties].[博德特氏菌属革兰氏阴性菌各种结构成分的分离及其性质研究。II. 百日咳博德特氏菌原生质体细胞质膜组分的分离及其化学和酶学性质研究]
Zh Mikrobiol Epidemiol Immunobiol. 1976 Oct(10):88-94.
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Polymorphonuclear leukocyte-inhibitory factor of Bordetella pertussis. II. Localization in the outer membrane.百日咳博德特氏菌的多形核白细胞抑制因子。II. 在外膜中的定位。
Biken J. 1979 Mar;22(1):1-10.
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[Localization of toxic substances in microbial variants of B. pertussis with defective cell walls].[细胞壁缺陷型百日咳博德特氏菌微生物变体中有毒物质的定位]
Zh Mikrobiol Epidemiol Immunobiol. 1974 Oct(10):7-12.
10
[The chemical composition of the whole cell, cell wall and protoplasm of pertussis and parapertussis microbes].[百日咳杆菌和副百日咳杆菌微生物的全细胞、细胞壁及原生质的化学成分]
Zh Mikrobiol Epidemiol Immunobiol. 1967 Dec;44(12):79-83.