[从百日咳博德特氏菌中分离纯化限制性内切酶BpeI]

[Isolation and purification of restriction endonuclease BpeI from Bordetella pertussis].

作者信息

Kazennova E V, Tarasov A P, Mileikovskaia M M, Semina I E, Tsvetkova N V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1982 Jun(6):56-7.

Abstract

New restriction endonuclease has been isolated from Bordetella pertussis vaccine strain 305 and purified in 1 stage on Sepharose covalently bound with blue dextran. The isolated restrictase has been found capable of breaking down lambda-phag DNA into 7 fragments. According to its specificity, Bpe I is the isoschizomer of Hind III obtained from Haemophilus influenzae strain Rd.

摘要

新的限制性内切酶已从百日咳博德特氏菌疫苗株305中分离出来，并在与蓝色葡聚糖共价结合的琼脂糖凝胶上一步纯化。已发现分离出的限制酶能够将λ噬菌体DNA分解成7个片段。根据其特异性，Bpe I是从流感嗜血杆菌Rd株获得的Hind III的同裂酶。