Fogel R, Sharp G W, Donowitz M
Am J Physiol. 1982 Aug;243(2):G117-26. doi: 10.1152/ajpgi.1982.243.2.G117.
The effects of chloroquine diphosphate, a drug with "'membrane-stabilizing" properties, were studied on basal ileal absorption and on ileal secretion induced by increased intracellular cAMP levels and calcium (serotonin). The studies were performed on rat (in vivo) and rabbit ileum (in vitro). Intraluminal chloroquine (10(-4) M) reversed cholera toxin- and theophylline-induced secretion in rat ileum but did not alter the cholera toxin- and theophylline-induced increases in cAMP content. Addition of chloroquine (10(-4) M) to the mucosal surface of rabbit ileum did not alter basal active electrolyte transport or the serotonin-induced decreased Na and Cl absorption but inhibited the theophylline-induced C1 secretion. Addition of chloroquine (10(-4)) M) to the serosal surface stimulated net Na and Cl absorption. This effect may involve intracellular calcium. Chloroquine increased the rabbit ileal calcium content and decreased 45Ca2+ influx from the serosal surface. Both the mucosal and serosal effects of chloroquine described led to a net increase in absorptive function of the intestine and should prove useful in developing treatment of diarrheal diseases.
研究了具有“膜稳定”特性的药物磷酸氯喹对回肠基础吸收以及由细胞内环磷酸腺苷（cAMP）水平升高和钙（血清素）诱导的回肠分泌的影响。研究在大鼠（体内）和兔回肠（体外）上进行。肠腔内的氯喹（10⁻⁴M）可逆转大鼠回肠中霍乱毒素和茶碱诱导的分泌，但不会改变霍乱毒素和茶碱诱导的cAMP含量增加。向兔回肠黏膜表面添加氯喹（10⁻⁴M）不会改变基础活性电解质转运或血清素诱导的钠和氯吸收减少，但会抑制茶碱诱导的氯分泌。向浆膜表面添加氯喹（10⁻⁴M）会刺激钠和氯的净吸收。这种作用可能涉及细胞内钙。氯喹增加了兔回肠的钙含量，并减少了浆膜表面45Ca²⁺的内流。所描述的氯喹的黏膜和浆膜效应均导致肠道吸收功能的净增加，并且应该对开发腹泻病的治疗方法有用。
