Menestrina G, Antolini R
Biochim Biophys Acta. 1982 Jun 28;688(3):673-84. doi: 10.1016/0005-2736(82)90279-6.
Incorporation of Megatura crenulata hemocyanin into phosphatidylcholine black lipid membranes results in the formation of ion channels. Channel properties depend on many factors, three of which are examined in this work: type and concentration of electrolyte and applied voltage. Eight cations at different concentrations have been used. Instantaneous conductance of the channel is a saturating function of both applied voltage and ionic strength of the bathing solution with monovalent cations, but only of ionic strength with divalent cations. Steady-state voltage-conductance relations are nonlinear for both signs but show slight saturation with ionic strength. Relaxation towards the steady state can be fitted by two exponentials with different time constants. All experimental data are fitted postulating the existence of a mechanism of voltage gating of the channel, and of discrete negative charge near its mouth. Specific and nonspecific binding of cations is required.
将皱纹盘鲍血蓝蛋白掺入磷脂酰胆碱黑色脂质膜中会导致离子通道的形成。通道特性取决于许多因素，本研究考察了其中三个因素：电解质的类型和浓度以及施加的电压。使用了八种不同浓度的阳离子。通道的瞬时电导率对于施加电压和含有单价阳离子的浴液的离子强度而言是一个饱和函数，但对于含有二价阳离子的情况，仅与离子强度有关。稳态电压 - 电导关系对于两种符号都是非线性的，但随离子强度略有饱和。向稳态的弛豫可以用具有不同时间常数的两个指数函数来拟合。所有实验数据的拟合都假定通道存在电压门控机制以及其口部附近存在离散负电荷。需要阳离子的特异性和非特异性结合。
