Menestrina G, Pasquali F, Antolini R
Biophys Struct Mech. 1984;10(4):169-84. doi: 10.1007/BF00535546.
Helix pomatia hemocyanin forms ion-conducting channels in planar lipid bilayer membranes when added at mg/ml concentration. These channels have several original features. They fluctuate between one conducting and some poorly conducting states and fluctuations can be grouped in bursts. Different channels can have widely different conductance amplitudes. Both channel conductance and burst lifetime are dependent on the applied voltage. Fluctuations within a burst show a complex kinetic behaviour which has been explained developing a multistate model. The model calls for one single open state and six different closed states. Transitions are allowed only between one of the closed states and the open one and obey first order kinetics. This model is able to fit all our experimental curves obtained in single channel experiments.
将每毫克/毫升浓度的玛瑙螺血蓝蛋白添加到平面脂质双分子层膜中时，它会形成离子传导通道。这些通道具有几个独特的特征。它们在一个导通状态和一些导通性较差的状态之间波动，并且波动可以成串出现。不同的通道可能具有相差很大的电导幅度。通道电导和猝发寿命都取决于所施加的电压。猝发内的波动呈现出复杂的动力学行为，这一行为已通过建立多状态模型得到解释。该模型需要一个单一的开放状态和六个不同的关闭状态。转变仅允许在其中一个关闭状态和开放状态之间进行，并遵循一级动力学。这个模型能够拟合我们在单通道实验中获得的所有实验曲线。
