Le Hir M, Kaissling B, Dubach U C
Cell Tissue Res. 1982;224(3):493-504. doi: 10.1007/BF00213747.
Na-K-ATPase activity was measured in the convoluted part of the distal tubule (DCT), the connecting tubule (CNT) and the cortical collecting duct (CCD). The segments were microdissected from freeze-dried kidney tissue of rabbits adapted to various salt diets and exposed to large differences in endogenous and exogeneous mineralocorticoids. The Na-K-ATPase activity in the DCT is not influenced by mineralocorticoids. They do influence the activity in the CNT and in the CCD. In the CNT the highest activity was found with a low Na-, high K-diet. At the beginning of the CNT the enzyme activity is higher than in the end portion. While canrenoate-K treatment has no effect on Na-K-ATPase activity in the initial portions of the CNT, this drug decreases the Na-K-ATPase activity significantly in the end portion of the CNT. DOCA treatment has a significant effect on the enzyme activity in the CNT only in the end-portion of the segment, but provokes the highest Na-K-ATPase activity in the CCD. The changes in Na-K-ATPase are found to be associated with corresponding changes in the baso-lateral cell-membrane area in the segments affected.
在远曲小管（DCT）的曲部、连接小管（CNT）和皮质集合管（CCD）中测量钠钾ATP酶活性。这些节段是从适应不同盐饮食的兔冷冻干燥肾组织中显微解剖出来的，并暴露于内源性和外源性盐皮质激素的巨大差异中。DCT中的钠钾ATP酶活性不受盐皮质激素影响。它们确实会影响CNT和CCD中的活性。在CNT中，低钠高钾饮食时活性最高。在CNT起始部，酶活性高于末端部分。虽然坎利酸钾治疗对CNT起始部的钠钾ATP酶活性没有影响，但该药物会显著降低CNT末端部分的钠钾ATP酶活性。去氧皮质酮治疗仅在节段末端部分对CNT中的酶活性有显著影响，但会引发CCD中最高的钠钾ATP酶活性。发现钠钾ATP酶的变化与受影响节段基底外侧细胞膜面积的相应变化有关。
