ten Cate W J, Curtis L M, Rarey K E
Department of Anatomy, College of Medicine, University of Florida, Gainesville 32610.
Eur Arch Otorhinolaryngol. 1994;251(1):6-11. doi: 10.1007/BF00175950.
The effect of a low Na+, high K+ diet on Na+,K(+)-ATPase levels in cochlear lateral wall tissues was investigated in laboratory rats by using an enzyme-linked immunosorbent assay. The low Na+, high K+ diet induced high aldosterone plasma levels in the animals as well as changes in plasma cation levels. Animals that received a low Na+, high K+ diet demonstrated a statistically significant (97%) increase in Na+,K(+)-ATPase levels in the stria vascularis when compared to animals that received a control diet. This increase in strial Na+,K(+)-ATPase levels was blocked only 70% by administration of the aldosterone antagonist, spironolactone. Findings therefore indicate that strial Na+,K(+)-ATPase may be modulated by both aldosterone and Na+,K+ plasma levels. Na+,K(+)-ATPase levels in the spiral ligament were not affected by the experimental treatment. These findings suggest that spiral ligament Na+,K(+)-ATPase levels may be regulated by factors other than aldosterone and Na+,K+ plasma levels. This study provides further insight into the mechanisms of the beneficial effects of salt restriction and potassium loading in patients with Méniére's disease.
通过酶联免疫吸附测定法，在实验大鼠中研究了低钠高钾饮食对耳蜗外侧壁组织中钠钾ATP酶水平的影响。低钠高钾饮食导致动物体内醛固酮血浆水平升高以及血浆阳离子水平变化。与接受对照饮食的动物相比，接受低钠高钾饮食的动物的血管纹中钠钾ATP酶水平有统计学意义的升高（97%）。给予醛固酮拮抗剂螺内酯后，血管纹中钠钾ATP酶水平的这种升高仅被阻断了70%。因此，研究结果表明，血管纹中的钠钾ATP酶可能受醛固酮和血浆钠钾水平的调节。螺旋韧带中的钠钾ATP酶水平不受实验处理的影响。这些发现表明，螺旋韧带中的钠钾ATP酶水平可能受醛固酮和血浆钠钾水平以外的因素调节。本研究为梅尼埃病患者限盐和补钾有益作用的机制提供了进一步的见解。
