Shibasaki T, Masui H, Sato G, Ling N, Guillemin R
J Clin Endocrinol Metab. 1981 Feb;52(2):350-3. doi: 10.1210/jcem-52-2-350.
Fragments of the pituitary adenoma of a patient with Cushing's disease were maintained in defined culture medium. Immunoreactive (IR) ACTH, IR alpha-MSH, IR beta-lipotropin (beta-LPH), IR beta-endorphin, and IR gamma-MSHs secreted from the adenoma were studied with gel permeation chromatography and the respective RIAs. The adenoma secreted roughly equimolar quantities of IR beta-LPH plus IR beta-endorphin, IR gamma 3-MSHs, and IR ACTHs. It also secreted IR alpha-MSH as well as IR gamma 1-MSH, although in a much lower concentration than the above four peptides. The secreted gamma 3-MSH-like peptides were found to be glycosylated. The secretion pattern suggests that this particular adenoma processes the pro-opiomelanocortin molecule in pathways which encompass those of both the pars distalis and the pars intermedia.
将库欣病患者垂体腺瘤的碎片置于特定培养基中培养。采用凝胶渗透色谱法和相应的放射免疫分析法，对腺瘤分泌的免疫反应性（IR）促肾上腺皮质激素（ACTH）、IR α-促黑素（α-MSH）、IR β-促脂解素（β-LPH）、IR β-内啡肽和IR γ-促黑素（γ-MSHs）进行了研究。该腺瘤分泌的IR β-LPH加IR β-内啡肽、IR γ3-MSHs和IR ACTH的量大致相等。它还分泌IR α-MSH以及IR γ1-MSH，不过其浓度远低于上述四种肽。发现分泌的γ3-MSH样肽是糖基化的。这种分泌模式表明，该特定腺瘤在处理阿黑皮素原分子时所采用的途径既包括远侧部的途径，也包括中间部的途径。
