Lin T, Lincoln T M, Brown N, Murono E P, Osterman J, Nankin H R
Endocrinology. 1982 Oct;111(4):1391-3. doi: 10.1210/endo-111-4-1391.
Using a 0-32% continuous metrizamide density gradient, interstitial cells could be separated into five distinct bands. Cells localized in bands 1 (B1), 2 (B2), and 3 (B3) were isolated and incubated for 1h with or without human chorionic gonadotropin (hCG). Both B2 and B3 cells responded to hCG with increased cyclic AMP formation, but only B3 cells produced significantly more testosterone. Protein kinase activity of B2 cells was found to be extremely low compared with B1 and B3 cells. Additional treatment of B3 cells with collagenase did not cause any change in protein kinase activity. These results indicate that decreased protein kinase activity may be responsible for impaired testosterone synthesis in B2 cells.
使用0-32%的连续泛影葡胺密度梯度，间质细胞可被分离成五个不同的条带。将位于条带1（B1）、条带2（B2）和条带3（B3）中的细胞分离出来，并在有或无人绒毛膜促性腺激素（hCG）的情况下孵育1小时。B2和B3细胞对hCG的反应均表现为环磷酸腺苷生成增加，但只有B3细胞产生了显著更多的睾酮。与B1和B3细胞相比，发现B2细胞的蛋白激酶活性极低。用胶原酶对B3细胞进行额外处理并未导致蛋白激酶活性发生任何变化。这些结果表明，蛋白激酶活性降低可能是B2细胞中睾酮合成受损的原因。
