Lefèvre A, Saez J M, Finaz C
Horm Res. 1983;17(2):114-20. doi: 10.1159/000179685.
Collagenase dispersed cells from immature or mature rat testis were separated into 11 bands on a discontinuous Percoll gradient. Erythrocytes sedimented at the bottom. Only three bands (8, 9 and 10) bind efficiently [125I]hCG. In immature rats, cells in bands 8, 9 and 10 bind hCG with the same affinity, but differ slightly in their total number of sites (average 65,000 +/- 4,000 sites per Leydig cell) and in their cAMP and testosterone production in response to hCG. They present 8% of the total number of dispersed cells. In mature rat testis, populations 8, 9 and 10 differed markedly in their capacity to bind [125I]hCG and in their response to hCG as regards an increase in cAMP and testosterone production. Testosterone production was increased 2- and 17-fold in bands 8 and 10, respectively. In mature rats only 1.2% of the total cells are Leydig cells. In both mature and immature rats, band 10 is made of 100% Leydig cells, as all cells stained positively for 3 beta-hydroxysteroid dehydrogenase.
用胶原酶分散未成熟或成熟大鼠睾丸的细胞，在不连续的Percoll梯度上分离成11条带。红细胞沉淀在底部。只有三条带（8、9和10）能有效结合[125I]hCG。在未成熟大鼠中，带8、9和10中的细胞以相同的亲和力结合hCG，但它们的总结合位点数量（每个间质细胞平均65,000±4,000个位点）以及对hCG刺激的cAMP和睾酮产生略有不同。它们占分散细胞总数的8%。在成熟大鼠睾丸中，群体8、9和10在结合[125I]hCG的能力以及对hCG刺激的cAMP和睾酮产生增加的反应方面存在显著差异。带8和带10中的睾酮产生分别增加了2倍和17倍。在成熟大鼠中，间质细胞仅占总细胞数的1.2%。在成熟和未成熟大鼠中，带10均由100%的间质细胞组成，因为所有细胞对3β-羟基类固醇脱氢酶染色均呈阳性。
