Hasegawa R, Tatematsu M, Tsuda H, Shirai T, Hagiwara A, Ito N
Gan. 1982 Apr;73(2):264-9.
The effects of pre- and post-administration of 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB), benzo[a]pyrene (B[a]P), and phenobarbital (PB) on the induction of hyperplastic liver nodules by N-2-fluorenylacetamide (2-FAA) were studied in F344 rats. Rats were given 3'-Me-DAB (0.06% in the diet), B[a]P (80 mg/kg b.wt. x 7, intragastrically) or PB (0.05% in the diet) for 6 weeks before (experimental series I) or after (experimental series II) diet containing 0.02% 2-FAA for 2 weeks, and killed in week 10 of the experiment. The number and total area of hyperplastic nodules per unit area of liver were greatest in rats treated with 3'-Me-DAB in both experimental series. B[a]P significantly increased the induction of hyperplastic nodules in experimental series I and PB significantly increased it in experimental series II. Partial hepatectomy performed during administration of test chemicals enhanced the induction of hyperplastic nodules, especially in experimental series I, and an oral dose of carbon tetrachloride during 2-FAA feeding enhanced it in both experimental series. In the present system, initiating activity of both the hepato- and the non-hepatocarcinogen was detected, and promoting activity of both the hepatocarcinogen and the hepatopromoter was demonstrated.
在F344大鼠中研究了3'-甲基-4-二甲基氨基偶氮苯（3'-Me-DAB）、苯并[a]芘（B[a]P）和苯巴比妥（PB）在给予N-2-芴基乙酰胺（2-FAA）之前和之后对增生性肝结节诱导的影响。在含0.02% 2-FAA的饲料喂养2周之前（实验系列I）或之后（实验系列II），给大鼠喂食3'-Me-DAB（饲料中含0.06%）、B[a]P（80 mg/kg体重，经胃内给药，共7次）或PB（饲料中含0.05%），持续6周，并在实验的第10周处死。在两个实验系列中，用3'-Me-DAB处理的大鼠肝脏单位面积内增生性结节的数量和总面积最大。在实验系列I中，B[a]P显著增加了增生性结节的诱导，在实验系列II中，PB显著增加了增生性结节的诱导。在给予受试化学物质期间进行部分肝切除术增强了增生性结节的诱导，尤其是在实验系列I中，在2-FAA喂养期间口服四氯化碳在两个实验系列中均增强了增生性结节的诱导。在本系统中，检测到了肝致癌物和非肝致癌物的启动活性，并证明了肝致癌物和肝促癌剂的促进活性。
