Tatematsu M, Murasaki G, Nakanishi K, Miyata Y, Shinohara Y, Ito N
Gan. 1979 Feb;70(1):125-30.
Sequential quantitative analyses were made of hyperplastic liver nodules induced by treating male Fischer rats first with diethylnitrosamine (DEN) and then with N-(2-fluorenyl)acetamide (2-FAA), alpha-isomer of 1,2-3,4,5,6-hexachlorocyclohexane (alpha-BHC), or phenobarbital. The test rats were injected ip with 200 mg/kg body weight of DEN, given basal diet containing 2-FAA, alpha-BHC, or phenobarbital from week 2 to 12, and subjected to partial hepatectomy at the end of week 3. From week 6, significantly higher percentage areas of hyperplastic nodules per unit area of liver were found in all experimental groups than in the control group. From week 8, significantly more hyperplastic nodules were found in all experimental groups than in the control group. Continuous administration of alpha-BHC or phenobarbital for 12 weeks with partial hepatectomy did not induce hyperplastic nodules. Continuous administration of 2-FAA did induce hyperplastic nodules, but the percentage areas and number of these nodules were significantly lower than in rats injected with DEN and then given 2-FAA orally, with partial hepatectomy.
对雄性Fischer大鼠进行序贯定量分析，这些大鼠首先用二乙基亚硝胺（DEN）处理，然后用N-（2-芴基）乙酰胺（2-FAA）、1,2,3,4,5,6-六氯环己烷的α异构体（α-BHC）或苯巴比妥处理，以诱导肝增生结节。给试验大鼠腹腔注射200mg/kg体重的DEN，从第2周到第12周给予含2-FAA、α-BHC或苯巴比妥的基础饮食，并在第3周结束时进行部分肝切除术。从第6周起，所有实验组肝脏单位面积内增生结节的百分比面积均显著高于对照组。从第8周起，所有实验组的增生结节均显著多于对照组。在部分肝切除术后连续12周给予α-BHC或苯巴比妥并未诱导增生结节。连续给予2-FAA确实诱导了增生结节，但其结节的百分比面积和数量显著低于先注射DEN然后口服2-FAA并进行部分肝切除术的大鼠。
