Ito N, Tatematsu M, Nakanishi K, Hasegawa R, Takano T, Imaida K, Ogiso T
Gan. 1980 Dec;71(6):832-42.
The effects of various hepatocarcinogenic, non-hepatocarcinogenic and non-carcinogenic chemicals on the induction of hyperplastic liver nodules by N-nitrosodiethylamine (DEN) or N-2-fluorenylacetamide (2-FAA) as an initiator were studied in male Fischer 344 rats. Rats were injected intraperitoneally with 200 mg of DEN/kg body weight or were fed on basal diet containing 200 ppm of 2-FAA for 2 weeks, and then given various test chemicals starting from week 3. They were also partially hepatectomized in week 3. All animals were killed at the end of week 8 and examined histologically. For quantitative analysis, hyperplastic nodules in the liver were measured with a color video image processor, VIP-21C. The effect of various chemicals in rats treated with DEN or with 2-FAA were compared. The production of hyperplastic liver nodules was greatest in rats treated with strong hepatocarcinogens, and less in rats treated with weak hepatocarcinogens. Very few hyperplastic nodules were produced after treatment with non-hepatocarcinogens or noncarcinogens. Hyperplastic nodules were formed in rats treated with phenobarbital, which is a hepatopromoter. Saccharin, which is a urinary bladder promoter, did not enhance the production of hyperplastic nodules in the liver. These results indicate that many hepatocarcinogens enhance liver carcinogenesis. The classification of chemicals as liver carcinogens is discussed on the basis of the results.
在雄性Fischer 344大鼠中，研究了各种致肝癌、非致肝癌和非致癌化学物质对以N-亚硝基二乙胺（DEN）或N-2-芴基乙酰胺（2-FAA）作为引发剂诱导增生性肝结节的影响。给大鼠腹腔注射200mg/kg体重的DEN，或在基础饲料中添加200ppm的2-FAA喂养2周，然后从第3周开始给予各种受试化学物质。在第3周时还对它们进行了部分肝切除术。所有动物在第8周结束时处死并进行组织学检查。为了进行定量分析，用彩色视频图像处理器VIP-21C测量肝脏中的增生性结节。比较了用DEN或2-FAA处理的大鼠中各种化学物质的作用。用强致癌物处理的大鼠中增生性肝结节的产生最多，用弱致癌物处理的大鼠中较少。用非致癌物或非致癌物质处理后产生的增生性结节很少。在用作为肝促癌剂的苯巴比妥处理的大鼠中形成了增生性结节。作为膀胱促癌剂的糖精并没有增加肝脏中增生性结节的产生。这些结果表明许多致癌物会增强肝癌发生。根据这些结果讨论了化学物质作为肝脏致癌物的分类。
