通过自旋标记研究血红蛋白伊豆（猕猴）与有机磷酸盐之间的相互作用。

Study on the interaction between hemoglobin Izu (Macaca) and organic phosphates by spin labeling.

作者信息

Nakayama S, Hoshino M, Takenaka O, Takahashi K, Watanabe T, Yoshida M

出版信息

J Biochem. 1982 Jun;91(6):1995-8. doi: 10.1093/oxfordjournals.jbchem.a133892.

DOI:10.1093/oxfordjournals.jbchem.a133892

Abstract

ESR spectra of the carbonmonoxy, oxy, and deoxy derivatives of hemoglobin Izu [Hb Izu (Macaca): beta 83 (EF 7) Gly leads to Cys] labeled at cysteine beta 83 with maleimide spin label have been observed in the presence and absence of 2,3-diphosphoglycerate and inositol hexaphosphate. The tau c values obtained from the spectra indicated that inositol hexaphosphate binds to all the derivatives of Hb Izu, but 2,3-diphosphoglycerate only to the deoxy derivatives.

摘要

在有和没有2,3 - 二磷酸甘油酸酯及肌醇六磷酸的情况下，观察了用马来酰亚胺自旋标记对半胱氨酸β83进行标记的血红蛋白伊豆[Hb伊豆（猕猴）：β83（EF 7）甘氨酸突变为半胱氨酸]的一氧化碳、氧和脱氧衍生物的电子自旋共振光谱。从光谱获得的τc值表明，肌醇六磷酸与Hb伊豆的所有衍生物结合，但2,3 - 二磷酸甘油酸酯仅与脱氧衍生物结合。