一氧化碳镰状血红蛋白分子间相互作用的自旋标记检测
Spin label detection of intermolecular interactions in carbonmonoxy sickle hemoglobin.
作者信息
Johnson M E, Danyluk S S
出版信息
Biophys J. 1978 Nov;24(2):517-24. doi: 10.1016/S0006-3495(78)85398-3.
Abstract
With recently developed spin label techniques for monitoring macromolecular rotational motion, heme-liganded sickle cell hemoglobin in the presence of inositol hexaphosphate is shown to exhibit restricted motional freedom as compared to liganded normal adult human hemoglobin. This motional restriction is dependent on both hemoglobin concentration and temperature.
摘要
利用最近开发的用于监测大分子旋转运动的自旋标记技术，研究发现，与配体化的正常成人血红蛋白相比，在存在肌醇六磷酸的情况下，血红素配体化的镰状细胞血红蛋白表现出受限的运动自由度。这种运动受限取决于血红蛋白浓度和温度。
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