Edwards C, Chance B
J Gen Microbiol. 1982 Jul;128(7):1409-14. doi: 10.1099/00221287-128-7-1409.
Increasing concentrations of cyanide inhibited the respiration of whole cells of Crithidia oncopelti in a biphasic fashion. Approximately 80% inhibition was attained with 40 microM-KCN. No further inhibition occurred until the concentration of KCN reached approximately 200 microM. Thereafter inhibition rose gradually to 100% at 1500 microM-KCN. Difference spectra revealed the presence of two CO-reacting haemoproteins. These were shown to be two different functional oxidases by photochemical action spectra obtained by using laser light. One oxidase was identified as cytochrome a + a3 whilst the other had the properties of cytochrome o. Both oxidases could be detected in cells at all stages of growth by the above methods.
氰化物浓度的增加以双相方式抑制了罗氏锥虫全细胞的呼吸作用。40微摩尔/升的氰化钾可导致约80%的抑制率。在氰化钾浓度达到约200微摩尔/升之前，抑制作用不再增强。此后，抑制率逐渐上升，在1500微摩尔/升的氰化钾时达到100%。差示光谱显示存在两种与一氧化碳反应的血红蛋白。通过激光光化学作用光谱表明，它们是两种不同的功能性氧化酶。一种氧化酶被鉴定为细胞色素a + a3，而另一种具有细胞色素o的特性。通过上述方法，在细胞生长的各个阶段都能检测到这两种氧化酶。
