Edwards S W, Lloyd D
Department of Biochemistry, University of Liverpool.
Biosci Rep. 1987 Mar;7(3):193-9. doi: 10.1007/BF01124789.
Room temperature, CO-difference spectra of intact rat polymorphonuclear leucocytes (neutrophils) revealed the presence of a number of CO-binding haemoproteins. Absorption maxima at 413, 540 and 570 nm were attributed to the CO-complex of cytochrome b-245 whereas an absorption maximum at 595 nm was assigned to the contribution from a myeloperoxidase complex, since an identical absorption maximum was observed in CO-difference spectra of purified myeloperoxidase in the presence of H2O2. Photochemical action spectra for the relief of CO-inhibited O2 uptake revealed contributions from both cytochrome b-245 and myeloperoxidase. The potential of these two O2- and CO-binding haemoproteins to function as oxidases during the respiratory burst is discussed.
完整大鼠多形核白细胞（中性粒细胞）的室温一氧化碳差异光谱显示存在多种与一氧化碳结合的血红蛋白。413、540和570纳米处的吸收最大值归因于细胞色素b - 245的一氧化碳复合物，而595纳米处的吸收最大值则归因于髓过氧化物酶复合物的贡献，因为在过氧化氢存在下纯化的髓过氧化物酶的一氧化碳差异光谱中观察到了相同的吸收最大值。缓解一氧化碳抑制的氧气摄取的光化学作用光谱显示细胞色素b - 245和髓过氧化物酶都有贡献。讨论了这两种与氧气和一氧化碳结合的血红蛋白在呼吸爆发期间作为氧化酶发挥作用的潜力。
