大鼠脑中胆碱激酶或ATP柠檬酸裂解酶与胆碱能活性的非相关性

Noncorrelation of choline kinase or ATP citrate lyase with cholinergic activity in rat brain.

作者信息

Harvey S A, Leong S F, Clark J B

出版信息

J Neurochem. 1982 Nov;39(5):1481-4. doi: 10.1111/j.1471-4159.1982.tb12595.x.

DOI:10.1111/j.1471-4159.1982.tb12595.x

Abstract

The activity of choline acetyltransferase was used as an index of cholinergic structures in regions of rat brain. The activities of ATP citrate lyase and choline kinase correlated poorly with cholinergic activity in whole tissue fractions, contrasting with the good correlation between acetylcholinesterase and choline acetyltransferase. Choline acetyltransferase was preferentially localised in synaptosomes prepared from regions of high (striatum) or intermediate (cortex, medulla oblongata/pons) cholinergic activity. In general, this was not true for either choline kinase or ATP citrate lyase.

摘要

胆碱乙酰转移酶的活性被用作大鼠脑区胆碱能结构的指标。在全组织组分中，ATP柠檬酸裂解酶和胆碱激酶的活性与胆碱能活性的相关性较差，这与乙酰胆碱酯酶和胆碱乙酰转移酶之间的良好相关性形成对比。胆碱乙酰转移酶优先定位于从高胆碱能活性区域（纹状体）或中等胆碱能活性区域（皮质、延髓/脑桥）制备的突触体中。一般来说，胆碱激酶或ATP柠檬酸裂解酶并非如此。