Garverick H A, Polge C, Flint A P
J Reprod Fertil. 1982 Sep;66(1):371-7. doi: 10.1530/jrf.0.0660371.
Occupied and unoccupied LH receptors in corpora lutea, and LH and progesterone concentrations in circulating plasma, were measured in non-pregnant gilts that had been treated with oestradiol-17 beta benzoate to prolong luteal function. Oestradiol benzoate (5 mg, administered on Day 12 after oestrus) delayed luteal regression and the decline in LH receptor levels at luteolysis and raised unoccupied receptor levels from 11.8 +/- 1.14 fmol/mg protein on Days 10--15 after oestrus to 31.8 +/- 3.26 fmol/mg protein on Days 15--21. There was no simultaneous rise in occupied receptor levels and occupancy decreased from 29.8 +/- 3.01 to 11.5 +/- 1.26%. Basal plasma LH concentrations were unchanged by oestradiol, but mean corpus luteum weight and plasma progesterone concentrations were slightly reduced. Oestradiol benzoate on Day 12 caused a similar increase in unoccupied receptor levels in gilts hysterectomized on Days 6--9 after oestrus, from 17.0 +/- 5.83 to 34.5 +/- 6.00 fmol/mg protein, determined on Days 15--18. Plasma concentrations of LH and progesterone were unchanged by oestradiol. Unoccupied receptor levels in corpora lutea and plasma LH and progesterone were unaltered by hysterectomy in untreated gilts. Occupied receptor levels were not influenced by hysterectomy or oestradiol. It is concluded that oestradiol-17 beta raises luteal LH receptor levels by a mechanism independent of the uterus.
在经17β-雌二醇苯甲酸酯处理以延长黄体功能的未孕后备母猪中，测定了黄体中被占据和未被占据的促黄体生成素（LH）受体，以及循环血浆中的LH和孕酮浓度。苯甲酸雌二醇（5毫克，在发情后第12天给药）延迟了黄体退化以及黄体溶解时LH受体水平的下降，并使未被占据的受体水平从发情后第10 - 15天的11.8±1.14飞摩尔/毫克蛋白升高至第15 - 21天的31.8±3.26飞摩尔/毫克蛋白。被占据的受体水平没有同时升高，占有率从29.8±3.01%降至11.5±1.26%。基础血浆LH浓度未因雌二醇而改变，但平均黄体重量和血浆孕酮浓度略有降低。发情后第12天给予苯甲酸雌二醇，在发情后第6 - 9天进行子宫切除的后备母猪中，未被占据的受体水平也有类似升高，从第15 - 18天测定的17.0±5.83飞摩尔/毫克蛋白升至34.5±6.00飞摩尔/毫克蛋白。LH和孕酮的血浆浓度未因雌二醇而改变。在未处理的后备母猪中，子宫切除对黄体中未被占据的受体水平以及血浆LH和孕酮没有影响。被占据的受体水平不受子宫切除或雌二醇的影响。结论是，17β-雌二醇通过一种独立于子宫的机制提高黄体LH受体水平。
