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DNA的定点补骨脂素交联

Site-directed psoralen crosslinking of DNA.

作者信息

Saffran W A, Goldenberg M, Cantor C R

出版信息

Proc Natl Acad Sci U S A. 1982 Aug;79(15):4594-8. doi: 10.1073/pnas.79.15.4594.

DOI:10.1073/pnas.79.15.4594
PMID:6289307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346721/
Abstract

A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specifically directed to this site in the dark through a Hg-S linkage prior to irradiation to form a crosslink. The location of interstrand cross-links was examined by electron microscopy of Pst I-cut molecules and found to be at or near the BamHI site.

摘要

已开发出一种通过使用含巯基的补骨脂素衍生物将位点特异性交联引入DNA的技术。质粒pBR322在独特的BamHI限制性位点处产生切口，并通过切口平移引入汞化嘧啶。然后在照射形成交联之前，补骨脂素在黑暗中通过Hg-S键被特异性地导向该位点。通过对Pst I切割分子的电子显微镜检查来检测链间交联的位置，发现其位于BamHI位点处或附近。

相似文献

1
Site-directed psoralen crosslinking of DNA.DNA的定点补骨脂素交联
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4594-8. doi: 10.1073/pnas.79.15.4594.
2
The complete pattern of mutagenesis arising from the repair of site-specific psoralen crosslinks: analysis by oligonucleotide hybridization.由位点特异性补骨脂素交联修复产生的诱变完整模式：通过寡核苷酸杂交进行分析。
Nucleic Acids Res. 1984 Dec 21;12(24):9237-48. doi: 10.1093/nar/12.24.9237.
3
Mutagenic SOS repair of site-specific psoralen damage in plasmid pBR322.质粒pBR322中位点特异性补骨脂素损伤的诱变SOS修复
J Mol Biol. 1984 Sep 25;178(3):595-609. doi: 10.1016/0022-2836(84)90240-7.
4
Marking the polarity of RNA molecules for electron microscopy by covalent attachment of psoralen-DNA restriction fragments.通过补骨脂素-DNA限制性片段的共价连接标记用于电子显微镜观察的RNA分子的极性
Proc Natl Acad Sci U S A. 1982 Jul;79(13):3940-4. doi: 10.1073/pnas.79.13.3940.
5
Site specificity of psoralen-DNA interstrand cross-linking determined by nuclease Bal31 digestion.
Biochemistry. 1986 Oct 21;25(21):6598-603. doi: 10.1021/bi00369a039.
6
Mapping the location of psoralen crosslinks on RNA by mung bean nuclease sensitivity of RNA.DNA hybrids.通过RNA-DNA杂交体对绿豆核酸酶的敏感性来定位补骨脂素交联在RNA上的位置。
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1381-5. doi: 10.1073/pnas.82.5.1381.
7
Psoralen-cross-linking study of the organization of intracellular adenovirus nucleoprotein complexes.补骨脂素交联法研究细胞内腺病毒核蛋白复合物的组织形式
J Virol. 1988 Apr;62(4):1227-34. doi: 10.1128/JVI.62.4.1227-1234.1988.
8
Psoralen-crosslinking of DNA as a probe for the structure of active nucleolar chromatin.补骨脂素交联DNA作为活性核仁染色质结构的一种探针。
J Mol Biol. 1984 Oct 5;178(4):897-919. doi: 10.1016/0022-2836(84)90318-8.
9
Synthesis and properties of novel psoralen derivatives.
Biochemistry. 1988 Sep 6;27(18):6971-6. doi: 10.1021/bi00418a045.
10
On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study.
Photochem Photobiol. 1988 Nov;48(5):643-6. doi: 10.1111/j.1751-1097.1988.tb02875.x.

引用本文的文献

1
Neighborhood of 16S rRNA nucleotides U788/U789 in the 30S ribosomal subunit determined by site-directed crosslinking.通过定点交联确定的30S核糖体亚基中16S rRNA核苷酸U788/U789的邻近区域。
RNA. 1998 Nov;4(11):1373-85. doi: 10.1017/s1355838298981134.
2
The complete pattern of mutagenesis arising from the repair of site-specific psoralen crosslinks: analysis by oligonucleotide hybridization.由位点特异性补骨脂素交联修复产生的诱变完整模式：通过寡核苷酸杂交进行分析。
Nucleic Acids Res. 1984 Dec 21;12(24):9237-48. doi: 10.1093/nar/12.24.9237.
3
Preparation and characterization of biotinylated psoralen.

本文引用的文献

1
Inter-strand cross-linkages occurring in the photoreaction between psoralen and DNA.补骨脂素与DNA光反应中发生的链间交联。
FEBS Lett. 1970 Jul 29;9(2):121-123. doi: 10.1016/0014-5793(70)80330-1.
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Nucleic Acids Res. 1988 Aug 11;16(15):7221-31. doi: 10.1093/nar/16.15.7221.
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Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.使用螯合基团取代补骨脂素标记的DNA探针进行灵敏的非放射性斑点杂交以及通过铕离子荧光进行定量检测。
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Specificity of site directed psoralen addition to RNA.定点向RNA添加补骨脂素的特异性
Nucleic Acids Res. 1989 May 11;17(9):3359-72. doi: 10.1093/nar/17.9.3359.
6
The structure of a pre-mRNA molecule in solution determined with a site directed cross-linking reagent.用位点定向交联试剂测定溶液中前体mRNA分子的结构。
Nucleic Acids Res. 1990 Feb 25;18(4):855-64. doi: 10.1093/nar/18.4.855.
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A protected biotin containing deoxycytidine building block for solid phase synthesis of biotinylated oligonucleotides.一种用于生物素化寡核苷酸固相合成的含脱氧胞苷构建单元的保护生物素。
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BamHI核酸内切酶的纯化及性质
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Determination of sulfhydryl groups with 2,2'- or 4,4'-dithiodipyridine.用2,2'-或4,4'-二硫代二吡啶测定巯基
Arch Biochem Biophys. 1967 Mar;119(1):41-9. doi: 10.1016/0003-9861(67)90426-2.
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The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.噬菌体T5的染色体。I. 用琼脂糖凝胶电泳分析单链DNA片段
J Mol Biol. 1972 Feb 14;63(3):383-95. doi: 10.1016/0022-2836(72)90435-4.
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Light-induced cross-linking of DNA in the presence of a furocoumarin (psoralen). Studies with phage lambda, Escherichia coli, and mouse leukemia cells.在呋喃香豆素（补骨脂素）存在的情况下，光诱导DNA交联。用λ噬菌体、大肠杆菌和小鼠白血病细胞进行的研究。
Biochim Biophys Acta. 1970 Sep 17;217(1):30-9. doi: 10.1016/0005-2787(70)90119-x.
8
Electron microscopy of DNA crosslinked with trimethylpsoralen: test of the secondary structure of eukaryotic inverted repeat sequences.与三甲基补骨脂素交联的DNA的电子显微镜观察：真核生物反向重复序列二级结构的检测
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2644-8. doi: 10.1073/pnas.73.8.2644.
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Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.具有与DNA和RNA更高光反应活性的新型补骨脂素衍生物的合成与表征
Biochemistry. 1977 Mar 22;16(6):1058-64. doi: 10.1021/bi00625a005.
10
Photochemical production of psoralen - DNA monoadducts capable of subsequent photocrosslinking.能够进行后续光交联的补骨脂素-DNA单加合物的光化学产生。
Nucleic Acids Res. 1978 Oct;5(10):3619-33. doi: 10.1093/nar/5.10.3619.