Chatterjee P K, Cantor C R
Nucleic Acids Res. 1978 Oct;5(10):3619-33. doi: 10.1093/nar/5.10.3619.
Irradiation of 4' -aminomethyl-4,5' ,8-trimethyl psoralen in the presence of DNA at wavelengths between 380 and 400nm leads to efficient production of monoadducts with no significant amount of crosslinking. The yield of monoadduct attainable is high (about 35 adducts per 1000 base pairs) and can be made still higher by repeated irradiation with fresh psoralen derivative. Subsequent irradiation at 350nm results in crosslinking of a almost half of the monoattached psoralen. Studies of the yield of nonoadduct and crosslink as function of irradiation wavelength show that the action spectrum for crosslink formation is blue-shifted relative to that for monoadduct formation.
在波长为380至400纳米的条件下，于DNA存在时对4'-氨甲基-4,5',8-三甲基补骨脂素进行辐照，可高效生成单加合物，且交联量极少。可达到的单加合物产量很高（每1000个碱基对约有35个加合物），通过用新鲜的补骨脂素衍生物反复辐照，产量还可进一步提高。随后在350纳米处进行辐照，会使几乎一半单附着的补骨脂素发生交联。对非加合物和交联产量随辐照波长变化的研究表明，交联形成的作用光谱相对于单加合物形成的作用光谱发生了蓝移。
