Bailey C L, Faran M E, Gargan T P, Hayes D E
Am J Trop Med Hyg. 1982 Sep;31(5):1054-61. doi: 10.4269/ajtmh.1982.31.1054.
Comparisons were made between groups of Culex pipiens L. with different physiologic histories to test their ability to sucessfully overwinter under field conditions. On 14 December 1978, each group of mosquitoes was marked with a distinctive fluorescent dust and released inside an abandoned ammunition bunker at Fort Washington, Maryland. To insure that dead mosquitoes could be dissected and information obtained on their ovarian development, a sample of females from each group was also released into a plexiglass cage that was attached to the inside wall of the room. The physiologic histories of each group of mosquitoes were as follows: (a) "wild caught", those which had entered the bunker prior to the release date, (b) "lab-reared diapausing nonblood-fed," (c) "lab-reared diapausing blood-fed nongravid, " (d)"lab-reared diapausing blood-fed gravid," (e) "lab-reared nondiapausing nonblood-fed," and (f) "lab-reared nondiapausing blood-fed." By 8 March 1979, all of the lab-reared nondiapausing groups, of mosquitoes released in the room had died, whereas 15.7, 22.4 and 24.7% were recovered from the "lab-reared diapausing nonblood-fed," "lab-reared diapausing blood-fed" (gravid and nongravid) and "wild caught" mosquitoes, respectively. For the mosquitoes in the cage, only 0, 2.1 and 7.0% of the "lab-reared nondiapausing blood-fed," "lab-reared nondiapausing nonblood-fed" and "lab-reared diapausing blood-fed gravid," respectively, survived. This compared to 45.4, 56.8 and 58.0%, respectively, for the "lab-reared diapausing nonblood-fed," "lab-reared diapausing blood-fed nongravid" and the "wild caught" groups. These data provide evidence to support the theory that a significant number of diapausing Cx. pipiens which have taken a prehibernation (possibly viremic) blood meal do not develop eggs and can survive the winter at rates comparable to diapausing nonblood-fed mosquitoes.
对具有不同生理史的尖音库蚊群体进行了比较，以测试它们在野外条件下成功越冬的能力。1978年12月14日，每组蚊子都用一种独特的荧光粉尘进行标记，然后释放到马里兰州华盛顿堡一个废弃的弹药库内。为确保能够解剖死亡的蚊子并获取其卵巢发育的信息，每组中的一部分雌蚊样本也被释放到一个固定在室内墙壁上的有机玻璃笼中。每组蚊子的生理史如下：(a)“野外捕获的”，即在释放日期之前进入弹药库的蚊子；(b)“实验室饲养的滞育未吸血的”；(c)“实验室饲养的滞育吸血未孕的”；(d)“实验室饲养的滞育吸血已孕的”；(e)“实验室饲养的非滞育未吸血的”；(f)“实验室饲养的非滞育吸血的”。到1979年3月8日，释放到室内的所有实验室饲养的非滞育蚊子群体都已死亡，而分别从“实验室饲养的滞育未吸血的”、“实验室饲养的滞育吸血的（已孕和未孕）”和“野外捕获的”蚊子中回收了15.7%、22.4%和24.7%。对于笼中的蚊子，“实验室饲养的非滞育吸血的”、“实验室饲养的非滞育未吸血的”和“实验室饲养的滞育吸血已孕 的”分别只有0%、2.1%和7.0%存活。相比之下，“实验室饲养的滞育未吸血的”、“实验室饲养的滞育吸血未孕的”和“野外捕获的”群体的存活率分别为45.4%、56.8%和58.0%。这些数据提供了证据，支持以下理论：大量已进入滞育状态且在越冬前（可能处于病毒血症期）吸食过血的尖音库蚊不会发育卵巢，并且能够以与滞育未吸血蚊子相当的比率存活过冬。
