Mitchell C J, Briegel H
J Med Entomol. 1989 Jul;26(4):318-26. doi: 10.1093/jmedent/26.4.318.
Diapausing Culex pipiens L. females fed 10% sucrose for 7 d following eclosion contained significantly more lipids (P less than 0.05) than nondiapausing females reared and maintained at the same temperature (22 degrees C) but at a longer photophase (14:10 [L:D] instead of 9:15). Diapausing females with limited lipid reserves failed to increase their reserves after blood feeding. The average lipid content of 56 females tested decreased significantly (P less than 0.05) by day 6 after feeding and there was no correlation (r = -0.06) between lipid content at this time and original bloodmeal volumes of individual females. These results refute the contention that blood meals taken by diapausing Cx. pipiens result in fat body development when females are incubated at 18 degrees C during bloodmeal digestion. Diapausing Cx. pipiens with limited lipid reserves were unable to obtain sufficient energy from a single blood meal to survive extended hibernation. Although none became gravid, only 50% remained alive after 20 d in hibernation. In contrast, nonblood-fed females fed only 10% sucrose for 7 to 10 d before being placed in hibernation on a water diet survived for 6 mo with only 50% mortality. There was no evidence for gonotrophic dissociation. Failure of blood-fed, diapausing females to initiate vitellogenesis was correlated with the significantly smaller blood meals taken by most diapausing females and not with hypertrophy of the fat body or temperature during digestion.
羽化后以10%蔗糖为食7天的滞育致倦库蚊雌性个体，其体内脂质含量显著高于在相同温度（22摄氏度）下饲养和维持，但光照期更长（14:10[光照:黑暗]而非9:15）的非滞育雌性个体（P小于0.05）。脂质储备有限的滞育雌性个体在吸血后未能增加其储备。在吸血后第6天，所检测的56只雌性个体的平均脂质含量显著下降（P小于0.05），此时脂质含量与个体雌性最初的血餐量之间没有相关性（r = -0.06）。这些结果反驳了以下观点：滞育的致倦库蚊在血餐消化期间于18摄氏度下培育时，吸食血餐会导致脂肪体发育。脂质储备有限的滞育致倦库蚊无法从单次血餐中获取足够能量以度过漫长的冬眠期。尽管没有一只进入妊娠状态，但在冬眠20天后只有50%存活。相比之下，在以水为食进入冬眠前仅以10%蔗糖为食7至10天的未吸血雌性个体，6个月的存活率为50%。没有证据表明存在生殖营养分离。吸血的滞育雌性个体未能启动卵黄发生与大多数滞育雌性个体吸食的血餐显著较少有关，而与脂肪体肥大或消化期间的温度无关。
