A microneutralization test for the identification of enterovirus isolates.

Two-hundred and thirty-four enterovirus isolates were identified using a recently modified, microneutralization procedure. Neutralization tests were performed in 96 well plastic 'V' plates, using 20 microliter quantities of antisera and virus, then inoculated onto monolayers of buffalo green monkey kidney cells, growing in 48 well tissue culture plates. Utilization of this microneutralization procedure resulted in considerable savings of time, material, and particularly, neutralizing antisera.