分解中的鲣鱼（Katsuwonus pelamis）体内产生组胺的细菌。
Histamine-producing bacteria in decomposing skipjack tuna (Katsuwonus pelamis).
作者信息
Yoshinaga D H, Frank H A
出版信息
Appl Environ Microbiol. 1982 Aug;44(2):447-52. doi: 10.1128/aem.44.2.447-452.1982.
Abstract
Spoilage in skipjack tuna (Katsuwonus pelamis) was studied under controlled conditions by incubating whole, fresh fish in seawater at 38 degrees C, the optimum temperature for histamine formation. Bacterial isolates were obtained from the loin tissue of a decomposing tuna containing 134 mg of histamine per 100 g and a total anaerobic count of 3.5 x 10(5)/g after incubation for 24 h. Over 92% of the 134 isolates obtained were facultatively or obligately anaerobic bacteria. Eighteen isolates produced histamine in culture media containing histidine, and these were identified as Clostridium perfringens, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, and Vibrio alginolyticus. Histidine decarboxylase activity of several isolates was measured in a tuna broth medium and with resting cells suspended in a buffered histidine solution.
摘要
在38摄氏度（组胺形成的最适温度）的海水中对整条新鲜鲣鱼（Katsuwonus pelamis）进行控温培养，研究鲣鱼的腐败情况。从一条分解的金枪鱼的腰部组织中分离出细菌，该金枪鱼在培养24小时后，每100克含有134毫克组胺，总厌氧菌数为3.5×10⁵/g。从获得的134株分离菌中，超过92%为兼性或专性厌氧菌。18株分离菌在含有组氨酸的培养基中产生组胺，这些菌株被鉴定为产气荚膜梭菌、产气肠杆菌、肺炎克雷伯菌、奇异变形杆菌和溶藻弧菌。在金枪鱼肉汤培养基中以及将静止细胞悬浮于缓冲组氨酸溶液中，测定了几株分离菌的组氨酸脱羧酶活性。
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