用于估计猿猴病毒40感染滴度的形态学方法。
Morphological method for estimation of simian virus 40 infectious titer.
作者信息
Landau S M, Nosach L N, Pavlova G V
出版信息
Arch Virol. 1982;73(1):79-84. doi: 10.1007/BF01341731.
PMID:6289780
Abstract
The cytomorphologic method previously reported for titration of adenoviruses has been employed for estimating the infectious titer of simian virus 40 (SV 40). Infected cells forming intranuclear inclusions were determined. The method examined possesses a number of advantages over virus titration by plaque assay and cytopathic effect. The virus titer estimated by the method of inclusion counting and expressed as IFU/ml (Inclusion Forming Units/ml) corresponds to that estimated by plaque count and expressed as PFU/ml.
摘要
先前报道的用于腺病毒滴定的细胞形态学方法已被用于估计猿猴病毒40（SV 40）的感染滴度。确定形成核内包涵体的感染细胞。与通过空斑测定法和细胞病变效应进行病毒滴定相比，所研究的方法具有许多优点。通过包涵体计数法估计并以IFU/ml（包涵体形成单位/毫升）表示的病毒滴度与通过空斑计数法估计并以PFU/ml表示的病毒滴度相对应。
相似文献
1
Morphological method for estimation of simian virus 40 infectious titer.用于估计猿猴病毒40感染滴度的形态学方法。
Arch Virol. 1982;73(1):79-84. doi: 10.1007/BF01341731.
2
Activation of simian virus 40 by transfer of isolated chromosomes from transformed cells.通过转移来自转化细胞的分离染色体激活猴病毒40
Virology. 1974 Sep;61(1):303-5. doi: 10.1016/0042-6822(74)90266-9.
3
[Electron microscopic studies of kidney cells of Erythrocebus patas after infection with SV 40 and adenovirus type 12].[感染SV 40和12型腺病毒后赤猴肾细胞的电子显微镜研究]
Arch Gesamte Virusforsch. 1970;29(2):222-40.
4
Optimal conditions for titration of SV40 by the plaque assay method.用蚀斑试验法滴定SV40的最佳条件。
J Virol Methods. 1983 Aug;7(2):93-102. doi: 10.1016/0166-0934(83)90095-2.
5
Defective simian virus 40 genomes: isolation and growth of individual clones.缺陷型猿猴病毒40基因组：单个克隆的分离与生长
Virology. 1974 Nov;62(1):112-24. doi: 10.1016/0042-6822(74)90307-9.
6
Simian virus 40 production after viral uncoating in the CV-1 cell nucleus.猴病毒40在CV-1细胞核内脱壳后的产生情况。
J Virol. 1978 Oct;28(1):415-6. doi: 10.1128/JVI.28.1.415-416.1978.
7
Enhancement of adenovirus infection in WI-38 and AGMK cells by pretreatment of cells with 5-iododeoxyuridine.
Virology. 1975 Apr;64(2):513-9. doi: 10.1016/0042-6822(75)90128-2.
8
Spontaneous mutants of the adenovirus-simian virus 40 hybrid, Ad2+ND3, that grow efficiently in monkey cells.腺病毒-猴病毒40杂交体Ad2+ND3的自发突变体，其在猴细胞中能高效生长。
Virology. 1981 May;111(1):263-9. doi: 10.1016/0042-6822(81)90670-x.
9
Small plaque variants of simian virus 40 from a persistent infection of rhesus monkey kidney cells.
Virology. 1979 Aug;97(1):201-6. doi: 10.1016/0042-6822(79)90388-x.
10
Enhancement of the replication of human adenovirus in simian cells by a series of temperature-sensitive mutants of simian virus 40.
Virology. 1975 Jun;65(2):579-82. doi: 10.1016/0042-6822(75)90063-x.
引用本文的文献
1
Silencing of the rotavirus NSP4 protein decreases the incidence of biliary atresia in murine model.NSP4 蛋白的沉默可降低鼠模型胆道闭锁的发生率。
PLoS One. 2011;6(8):e23655. doi: 10.1371/journal.pone.0023655. Epub 2011 Aug 18.
本文引用的文献
1
An electron microscope study of the development of SV40 virus.猴空泡病毒40型（SV40）病毒发育的电子显微镜研究
J Cell Biol. 1963 May;17(2):423-41. doi: 10.1083/jcb.17.2.423.
2
The vacuolating virus, S.V. 40.空泡病毒，即猴病毒40（SV40）
Proc Soc Exp Biol Med. 1960 Nov;105:420-7. doi: 10.3181/00379727-105-26128.
3
Cytological and cytochemical studies of green monkey kidney cells infected in vitro with simian virus 40.对体外感染猿猴病毒40的绿猴肾细胞进行的细胞学和细胞化学研究。
J Cell Biol. 1965 Jun;25(3):529-43. doi: 10.1083/jcb.25.3.529.
4
Titration of adenovirus by counting cells containing virus-induced inclusion bodies.通过计数含有病毒诱导包涵体的细胞来滴定腺病毒。
Appl Microbiol. 1972 May;23(5):1025-6. doi: 10.1128/am.23.5.1025-1026.1972.
5
Expression of the chromosomal mouse Beta maj-globin gene cloned in SV40.克隆于SV40中的染色体小鼠β珠蛋白基因的表达。
Nature. 1979 Sep 6;281(5726):35-40. doi: 10.1038/281035a0.
6
Cloning the argF gene from Escherichia coli K-12 with simian virus 40.用猿猴病毒40从大肠杆菌K-12中克隆argF基因。
Gene. 1979 Sep;7(1):15-31. doi: 10.1016/0378-1119(79)90040-4.
7
Persistence of phage lambda DNA in genomes of mouse cells transformed by lambda-carrying SV40 vectors.携带λ噬菌体的SV40载体转化的小鼠细胞基因组中λ噬菌体DNA的持久性。
Gene. 1979 Jun;6(2):107-22. doi: 10.1016/0378-1119(79)90066-0.
8
Identification of simian virus 40 protein A.猿猴病毒40蛋白A的鉴定。
J Virol. 1977 Feb;21(2):636-46. doi: 10.1128/JVI.21.2.636-646.1977.
9
Morphological phenotype of temperature-sensitive mutants of simian virus 40 in productive infection.猿猴病毒40温度敏感突变体在增殖性感染中的形态学表型
Virology. 1977 Jun 15;79(2):355-68. doi: 10.1016/0042-6822(77)90363-4.
Zotero 插件