1,25-二羟胆钙化醇在人甲状腺髓样癌中的特异性结合
Specific binding of 1,25-dihydroxycholecalciferol in human medullary thyroid carcinoma.
作者信息
Freake H C, MacIntyre I
出版信息
Biochem J. 1982 Jul 15;206(1):181-4. doi: 10.1042/bj2060181.
Abstract
A specific 1,25-dihydroxycholecalciferol-binding protein has been detected in high-salt cytosols prepared from human medullary thyroid carcinomas. The binding protein had the same equilibrium dissociation constant (Kd = 0.17 +/- 0.05 nM; n = 4) and sedimentation coefficient on sucrose gradients (3.7S) as than seen in established vitamin D target tissues. This protein was not detected in normal thyroid cytosols, which may reflect the low proportion of C-cells within the gland.
摘要
在从人甲状腺髓样癌制备的高盐细胞溶质中检测到一种特异性的1,25 - 二羟胆钙化醇结合蛋白。该结合蛋白具有与已确定的维生素D靶组织中所见相同的平衡解离常数（Kd = 0.17±0.05 nM；n = 4）和在蔗糖梯度上的沉降系数（3.7S）。在正常甲状腺细胞溶质中未检测到这种蛋白，这可能反映了腺体中C细胞的比例较低。
相似文献
1
Specific binding of 1,25-dihydroxycholecalciferol in human medullary thyroid carcinoma.
Biochem J. 1982 Jul 15;206(1):181-4. doi: 10.1042/bj2060181.
2
The temporal distribution of the 1 alpha,25-dihydroxycholecalciferol receptor in the rat jejunal villus.
Clin Sci (Lond). 1984 Sep;67(3):285-90. doi: 10.1042/cs0670285.
3
Calcitriol receptors in rat thyroid follicular cells (FRTL-5).
Acta Endocrinol (Copenh). 1991 Nov;125(5):574-80. doi: 10.1530/acta.0.1250574.
4
Specific uptake of 1,25-dihydroxycholecalciferol by human chronic myeloid leukemia cells.
Cancer Res. 1984 Aug;44(8):3627-31.
5
Appearance of the rat testicular receptor for calcitriol (1,25-dihydroxyvitamin D3) during development.
J Steroid Biochem. 1985 Jul;23(1):51-6. doi: 10.1016/0022-4731(85)90259-6.
6
Binding of 1,25-dihydroxy-[3H]vitamin D3 in nuclear and cytosol fractions of whole mouse skin in vivo and in vitro.
FEBS Lett. 1981 Nov 16;134(2):203-6. doi: 10.1016/0014-5793(81)80602-3.
7
Sucrose density gradient analysis of 1,25-dihydroxyvitamin D3 binding in human breast tumors.
J Clin Endocrinol Metab. 1983 Apr;56(4):686-91. doi: 10.1210/jcem-56-4-686.
8
Androgen receptors in human thyroid tissue.
Surgery. 1984 Dec;96(6):996-1000.
9
A 1,25-dihydroxyvitamin D3 receptor-like protein in mammalian and avian liver nuclei.
Endocrinology. 1988 Jun;122(6):2584-9. doi: 10.1210/endo-122-6-2584.
10
Vitamin D receptors in isolated rat parotid gland acinar cells.
Biochim Biophys Acta. 1982 Oct 11;721(2):158-63. doi: 10.1016/0167-4889(82)90063-5.
引用本文的文献
1
Calcitriol and cancer therapy: A missed opportunity.
Bone Rep. 2018 Jun 13;9:110-119. doi: 10.1016/j.bonr.2018.06.002. eCollection 2018 Dec.
2
Calcitonin, the forgotten hormone: does it deserve to be forgotten?
Clin Kidney J. 2015 Apr;8(2):180-7. doi: 10.1093/ckj/sfv011. Epub 2015 Mar 20.
3
Increased incidence of medullary carcinoma of the thyroid in rural regions in Greece.
J Endocrinol Invest. 1995 Mar;18(3):220-3. doi: 10.1007/BF03347805.
4
Racial differences in calcitonin and katacalcin.
Calcif Tissue Int. 1984 Dec;36(6):725-8. doi: 10.1007/BF02405396.
5
Peptides from the calcitonin genes: molecular genetics, structure and function.
Biochem J. 1988 Oct 15;255(2):377-90. doi: 10.1042/bj2550377.
6
Regulation of calcitonin gene transcription by vitamin D metabolites in vivo in the rat.
J Clin Invest. 1988 Jan;81(1):270-3. doi: 10.1172/JCI113305.
7
Regulation of 1,25-dihydroxyvitamin D3 receptor gene expression by 1,25-dihydroxyvitamin D3 in the parathyroid in vivo.
J Clin Invest. 1990 Dec;86(6):1968-75. doi: 10.1172/JCI114931.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
CALCITONIN PRODUCTION AND THE MITOCHONDRION-RICH CELLS OF THE DOG THYROID.
Nature. 1964 Sep 5;203:1029-30. doi: 10.1038/2031029a0.
3
Biochemical evidence for 1,25-dihydroxyvitamin D receptor macromolecules in parathyroid, pancreatic, pituitary, and placental tissues.
Life Sci. 1980 Feb 4;26(5):407-14. doi: 10.1016/0024-3205(80)90158-7.
4
Calcitonin selectively stimulates 25-hydroxyvitamin D3-1 alpha-hydroxylase in proximal straight tubule of rat kidney.
Nature. 1981 May 28;291(5813):327-9. doi: 10.1038/291327a0.
5
Biochemical properties of 1 alpha, 25-dihydroxyvitamin D receptors.
J Steroid Biochem. 1980 Aug;13(8):977-89. doi: 10.1016/0022-4731(80)90173-9.
6
Stimulation of 1,25-dihydroxycholecalciferol production by prolactin and related peptides in intact renal cell preparations in vitro.
Biochim Biophys Acta. 1981 Jan 7;672(1):7-15. doi: 10.1016/0304-4165(81)90273-7.
7
Unoccupied 1,25-dihydroxyvitamin D3 receptors. Nuclear/cytosol ratio depends on ionic strength.
J Biol Chem. 1980 Jul 25;255(14):6799-805.
8
Thyroid cancer: tumour markers.
Recent Results Cancer Res. 1980;73:60-7.
9
Demonstration and organ distribution of the 1,25-dihydroxyvitamin D3-binding protein in fish (A. anguilla).
Endocrinology. 1982 Apr;110(4):1347-54. doi: 10.1210/endo-110-4-1347.
10
Purification of chicken intestinal receptor for 1 alpha, 25-dihydroxyvitamin D3 to apparent homogeneity.
Proc Natl Acad Sci U S A. 1982 Jan;79(1):16-20. doi: 10.1073/pnas.79.1.16.
Zotero 插件