Johnson M E, Lee L, Fung L W
Biochemistry. 1982 Aug 31;21(18):4459-67. doi: 10.1021/bi00261a041.
Model systems of cholestane and 5-doxylstearic acid analogue spin probes in lipid bilayer dispersions of dipalmitoylphosphatidylcholine and cholesterol (9:1 w/w) are used to analyze saturation transfer electron paramagnetic resonance spectral behavior for slow rotational diffusion in an anisotropic medium. Measurements are made at both 9 and 35 GHz to provide enhanced spectral resolution for different types of motion. Parameter correlation plots of spectral parameters from different regions of the saturation transfer spectra appear to be potentially useful in characterizing different types of motion. Anisotropic rotational diffusion about a symmetry axis coincident with the nitroxide y principal axis is clearly distinguishable from isotropic rotational diffusion and may be distinguishable from rotational diffusion about the nitroxide z principal axis. Approximate anisotropic rotational diffusion about a symmetry axis coincident with the nitroxide z principal axis is distinguishable from isotropic rotational diffusion under some, but not all, conditions.
使用胆甾烷和5-二氧硬脂酸类似物自旋探针在二棕榈酰磷脂酰胆碱和胆固醇（9:1 w/w）的脂质双层分散体中的模型系统，来分析在各向异性介质中慢旋转扩散的饱和转移电子顺磁共振光谱行为。在9 GHz和35 GHz下进行测量，以提供针对不同类型运动的增强光谱分辨率。来自饱和转移光谱不同区域的光谱参数的参数相关图似乎在表征不同类型的运动方面具有潜在用途。围绕与氮氧化物y主轴重合的对称轴的各向异性旋转扩散与各向同性旋转扩散明显不同，并且可能与围绕氮氧化物z主轴的旋转扩散不同。在某些但并非所有条件下，围绕与氮氧化物z主轴重合的对称轴的近似各向异性旋转扩散与各向同性旋转扩散是可区分的。
