Maziere C, Maziere J C, Mora L, Polonovski J
Biochim Biophys Acta. 1982 Sep 14;712(3):712-5. doi: 10.1016/0005-2760(82)90303-4.
In situ incorporation of two saturated (palmitic, 16:0; stearic, 18:0) and three unsaturated fatty acids (oleic, 18:1; linoleic, 18:2; arachidonic, 20:4) into the four major phospholipids, sphingomyelin, PC, PI and PE, was followed. Transformed cells incorporated unsaturated fatty acids more rapidly, whereas no significant differences were found concerning saturated fatty acids. In vitro determination of phospholipid acylation showed that incorporation of coenzyme A-activated forms of two saturated fatty acids (16:0 and 18:0) and one unsaturated fatty acid (18:1) into phospholipids was increased in transformed cells. Comparison of results obtained in situ and in vitro strongly suggests that incorporation of fatty acids into phospholipids in cultured cells is not limited by acyltransferase activities.
研究了两种饱和脂肪酸（棕榈酸，16:0；硬脂酸，18:0）和三种不饱和脂肪酸（油酸，18:1；亚油酸，18:2；花生四烯酸，20:4）原位掺入四种主要磷脂（鞘磷脂、磷脂酰胆碱、磷脂酰肌醇和磷脂酰乙醇胺）的情况。转化细胞掺入不饱和脂肪酸的速度更快，而在饱和脂肪酸方面未发现显著差异。磷脂酰化的体外测定表明，转化细胞中两种饱和脂肪酸（16:0和18:0）和一种不饱和脂肪酸（18:1）的辅酶A活化形式掺入磷脂的量增加。原位和体外获得的结果比较强烈表明，培养细胞中脂肪酸掺入磷脂不受酰基转移酶活性的限制。
