Mazière C, Mazière J C, Mora L, Auclair M, Polonovski J
Lipids. 1986 Aug;21(8):525-8. doi: 10.1007/BF02535641.
Incorporation of various exogenous saturated or unsaturated [14C]labeled fatty acids (palmitic, stearic, oleic, linoleic and arachidonic) into triacylglycerols by hamster fibroblasts was markedly enhanced (two- to fourfold) in the presence of theophylline or dibutyryl cyclic adenosine monophosphate (dbcAMP). This effect was observed for short-term (1-6 hr) as well as long-term (15-24 hr) preincubation with dbcAMP. In the presence of sodium fluoride, a phosphoprotein phosphatase inhibitor, measurement of diacylglycerol acyltransferase (DGAT) activity in cells pretreated with dbcAMP pointed out a marked increase (3 X) in specific activity. The results suggest that DGAT activity in fibroblasts could be activated by a cAMP-dependent phosphorylation process.
在茶碱或二丁酰环磷酸腺苷（dbcAMP）存在的情况下，仓鼠成纤维细胞将各种外源性饱和或不饱和的[14C]标记脂肪酸（棕榈酸、硬脂酸、油酸、亚油酸和花生四烯酸）掺入三酰甘油的能力显著增强（两到四倍）。在短期（1 - 6小时）以及长期（15 - 24小时）与dbcAMP预孵育时均观察到这种效应。在磷酸蛋白磷酸酶抑制剂氟化钠存在的情况下，对用dbcAMP预处理的细胞中的二酰甘油酰基转移酶（DGAT）活性进行测量，结果表明其比活性显著增加（3倍）。结果表明，成纤维细胞中的DGAT活性可能通过cAMP依赖性磷酸化过程被激活。
