受体枯草芽孢杆菌中存在的4,5',8-三甲基补骨脂素链间交联对转化DNA整合的影响。
Effect of 4,5',8-trimethylpsoralen interstrand cross-links present in recipient Bacillus subtilis on the integration of transforming DNA.
作者信息
Mooibroek H, van Randen J, Venema G
出版信息
J Bacteriol. 1982 Nov;152(2):669-75. doi: 10.1128/jb.152.2.669-675.1982.
Abstract
When recipient Bacillus subtilis carrying chromosomal trimethylpsoralen cross-links were transformed, the donor marker activity decreased with the extent of cross-linking. Additional donor marker activity was lost upon incubation of the reextracted DNA with nuclease S1, particularly at higher levels of cross-linking. Physical analysis of the reextracted DNA showed that the donor DNA was progressively excluded from heteroduplex formation as the frequency of cross-links in the recipient DNA increased. In the donor-recipient complexes still being formed, increasing amounts of donor DNA became susceptible to nuclease S1 digestion under these conditions. These results suggest that resident interstrand cross-links interfere both with initiation of recombination and with the completion of heteroduplex formation.
摘要
当携带染色体三甲基补骨脂素交联的受体枯草芽孢杆菌被转化时，供体标记活性随交联程度的增加而降低。重新提取的DNA与核酸酶S1孵育后，额外的供体标记活性丧失，尤其是在较高交联水平时。对重新提取的DNA进行物理分析表明，随着受体DNA中交联频率的增加，供体DNA逐渐被排除在异源双链体形成之外。在仍在形成的供体-受体复合物中，在这些条件下，越来越多的供体DNA变得易受核酸酶S1消化。这些结果表明，常驻链间交联既干扰重组的起始，也干扰异源双链体形成的完成。
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The effect of trimethylpsoralen--crosslinks on entry of donor DNA in transformation and transfection of Bacillus subtilis.三甲补骨脂素交联对供体DNA进入枯草芽孢杆菌转化和转染过程的影响。
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Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.感受态枯草芽孢杆菌摄取转化DNA后的命运。I. 供体-受体复合物的形成及特性
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