4,5',8-三甲基补骨脂素单加合物对转化型枯草芽孢杆菌脱氧核糖核酸的灭活作用
Inactivation of transforming Bacillus subtilis deoxyribonucleic acid by monoadducts of 4,5',8-trimethylpsoralen.
作者信息
te Riele H P, van Sluis P, Venema G
出版信息
J Bacteriol. 1981 Feb;145(2):832-9. doi: 10.1128/jb.145.2.832-839.1981.
4,5',8-Trimethylpsoralen (TMP) monoadducts inactive transforming deoxyribonucleic acid (DNA) in Bacillus subtilis. Contrary to TMP diadducts (TMP cross-links), which severely inhibit entry of donor DNA (G. Venema and U. Canosi, Mol. Gen. Genet. 179:1--11), TMP monoadducts have only a slight effect on entry. Since reextracted TMP-monoadduct-containing transforming DNA is a differentially repaired by Uvr- and Uvr+ recipients and cross-linkable to the recipient strand in the heteroduplex recombinant DNA molecules, the monoadducts can be integrated along with the donor DNA into the recipient chromosome.
4,5',8-三甲基补骨脂素（TMP）单加合物可使枯草芽孢杆菌中的转化脱氧核糖核酸（DNA）失活。与严重抑制供体DNA进入的TMP双加合物（TMP交联）相反（G. 韦内马和U. 卡诺西，《分子与普通遗传学》179:1 - 11），TMP单加合物对进入的影响很小。由于重新提取的含TMP单加合物的转化DNA在Uvr - 和Uvr + 受体中存在差异修复，并且在异源双链重组DNA分子中可与受体链交联，因此单加合物可与供体DNA一起整合到受体染色体中。
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