携带大肠杆菌乳糖基因的乳糖培养副溶血性弧菌的耐盐性
Salt tolerance of lactose-grown Vibrio parahaemolyticus carrying Escherichia coli lac genes.
作者信息
Datta A R, MacQuillan A M
出版信息
Appl Environ Microbiol. 1987 Feb;53(2):466-9. doi: 10.1128/aem.53.2.466-469.1987.
Abstract
Lac- strains of Vibrio parahaemolyticus were converted to Lac+ on receiving a hybrid plasmid containing the lactose utilization genes of Escherichia coli K-12. A V. parahaemolyticus strain containing this hybrid plasmid exhibited optimal growth rates on glucose and other carbon sources in the presence of 0.2 to 0.4 M NaCl. Growth of the same strain on lactose was inhibited at similar concentrations of NaCl. The altered growth rate responses in lactose medium appeared to be attributable to effects of NaCl on the activity of lactose permease, and possibly on that of beta-galactosidase, rather than on the levels of these enzymes in V. parahaemolyticus cells.
摘要
副溶血性弧菌的乳糖利用缺陷型菌株在获得含有大肠杆菌K-12乳糖利用基因的杂交质粒后转变为乳糖利用阳性型。含有这种杂交质粒的副溶血性弧菌菌株在0.2至0.4M氯化钠存在的情况下，在葡萄糖和其他碳源上表现出最佳生长速率。同一菌株在乳糖上的生长在相似浓度的氯化钠下受到抑制。乳糖培养基中生长速率的改变似乎归因于氯化钠对乳糖通透酶活性的影响，也可能对β-半乳糖苷酶活性的影响，而不是对副溶血性弧菌细胞中这些酶水平的影响。
相似文献
1
Salt tolerance of lactose-grown Vibrio parahaemolyticus carrying Escherichia coli lac genes.携带大肠杆菌乳糖基因的乳糖培养副溶血性弧菌的耐盐性
Appl Environ Microbiol. 1987 Feb;53(2):466-9. doi: 10.1128/aem.53.2.466-469.1987.
2
Possible mechanisms underlying the slow lactose fermentation phenotype in Shigella spp.志贺氏菌属中乳糖发酵缓慢表型潜在的机制
Appl Environ Microbiol. 1991 Oct;57(10):2912-7. doi: 10.1128/aem.57.10.2912-2917.1991.
3
Studies on the utilization of lactose by Corynebacterium glutamicum, bearing the lactose operon of Escherichia coli.对携带大肠杆菌乳糖操纵子的谷氨酸棒杆菌利用乳糖的研究。
Arch Microbiol. 1991;155(6):607-12. doi: 10.1007/BF00245357.
4
The lactose carrier of Escherichia coli functionally incorporated in Rhodopseudomonas sphaeroides obeys the regulatory conditions of the phototrophic bacterium.功能上整合到球形红假单胞菌中的大肠杆菌乳糖载体遵循光合细菌的调控条件。
FEBS Lett. 1983 Nov 28;164(1):185-90. doi: 10.1016/0014-5793(83)80046-5.
5
Highly efficient assimilation of lactose by a metabolically engineered strain of Saccharomyces cerevisiae.通过代谢工程改造的酿酒酵母菌株对乳糖的高效同化作用。
Yeast. 1998 Jun 30;14(9):827-37. doi: 10.1002/(SICI)1097-0061(19980630)14:9<827::AID-YEA281>3.0.CO;2-N.
6
The anomeric specificity of beta-galactosidase and lac permease from Escherichia coli.来自大肠杆菌的β-半乳糖苷酶和乳糖通透酶的异头特异性。
Can J Biochem. 1981 Feb;59(2):100-5. doi: 10.1139/o81-015.
7
Cloning of Escherichia coli lacZ and lacY genes and their expression in Gluconobacter oxydans and Acetobacter liquefaciens.大肠杆菌lacZ和lacY基因的克隆及其在氧化葡萄糖酸杆菌和液化醋杆菌中的表达。
Appl Environ Microbiol. 2002 May;68(5):2619-23. doi: 10.1128/AEM.68.5.2619-2623.2002.
8
Site-directed mutagenesis of cysteine-148 in the lac permease of Escherichia coli: effect on transport, binding, and sulfhydryl inactivation.大肠杆菌乳糖通透酶中半胱氨酸-148的定点诱变：对转运、结合及巯基失活的影响
Biochemistry. 1985 Dec 17;24(26):7628-35. doi: 10.1021/bi00347a020.
9
Substitution of glutamine-60 with glutamic acid causes the lac permease of Escherichia coli to become temperature sensitive.
Biochemistry. 1986 May 20;25(10):2778-81. doi: 10.1021/bi00358a006.
10
Autoregulation of lactose uptake through the LacY permease by enzyme IIAGlc of the PTS in Escherichia coli K-12.大肠杆菌K-12中磷酸转移酶系统的酶IIAGlc对通过LacY通透酶的乳糖摄取进行自动调节。
Mol Microbiol. 1999 Mar;31(6):1825-33. doi: 10.1046/j.1365-2958.1999.01319.x.
引用本文的文献
1
Molecular characterization of a novel glucosyltransferase from Lactobacillus reuteri strain 121 synthesizing a unique, highly branched glucan with alpha-(1-->4) and alpha-(1-->6) glucosidic bonds.来自罗伊氏乳杆菌121株的一种新型葡糖基转移酶的分子特征，该酶合成具有α-(1→4)和α-(1→6)糖苷键的独特、高度分支的葡聚糖。
Appl Environ Microbiol. 2002 Sep;68(9):4283-91. doi: 10.1128/AEM.68.9.4283-4291.2002.
本文引用的文献
1
Expression of a Lactose Transposon (Tn951) in Zymomonas mobilis.乳糖转座子（Tn951）在运动发酵单胞菌中的表达。
Appl Environ Microbiol. 1983 Nov;46(5):1163-8. doi: 10.1128/aem.46.5.1163-1168.1983.
2
A respiration-dependent primary sodium extrusion system functioning at alkaline pH in the marine bacterium Vibrio alginolyticus.一种在碱性pH值下发挥作用的、依赖呼吸作用的初级钠排出系统，存在于海洋细菌溶藻弧菌中。
Biochem Biophys Res Commun. 1981 Sep 16;102(1):265-71. doi: 10.1016/0006-291x(81)91516-3.
3
Roles of Na+ and K+ in alpha-aminoisobutyric acid transport by the marine bacterium Vibrio alginolyticus.钠离子和钾离子在海洋溶藻弧菌转运α-氨基异丁酸中的作用
J Biol Chem. 1982 Jan 25;257(2):788-94.
4
Vibrio parahaemolyticus and related halophilic Vibrios.副溶血性弧菌及相关嗜盐弧菌。
Crit Rev Microbiol. 1982;10(1):77-124. doi: 10.3109/10408418209113506.
5
Energetics of sodium-dependent alpha-aminoisobutyric acid transport in the moderate halophile Vibrio costicola.
Biochim Biophys Acta. 1984 Jul 27;766(1):77-87. doi: 10.1016/0005-2728(84)90219-6.
6
Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae.
J Bacteriol. 1983 Oct;156(1):19-29. doi: 10.1128/jb.156.1.19-29.1983.
7
Transposon mutagenesis of marine Vibrio spp.海洋弧菌属的转座子诱变
J Bacteriol. 1984 Jun;158(3):890-6. doi: 10.1128/jb.158.3.890-896.1984.
8
Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides.转座乳糖操纵子Tn951在球形红假单胞菌中的表达。
J Bacteriol. 1982 Nov;152(2):924-7. doi: 10.1128/jb.152.2.924-927.1982.
9
Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus.用于副溶血性弧菌这种海洋细菌的穿梭克隆载体。
J Bacteriol. 1984 Nov;160(2):808-11. doi: 10.1128/jb.160.2.808-811.1984.
10
The role of energy coupling in the transport of beta-galactosides by Escherichia coli.能量偶联在大肠杆菌转运β-半乳糖苷中的作用。
J Biol Chem. 1966 May 25;241(10):2200-11.
Zotero 插件