Bogdanova E S, Mirkin S M, Shmerling Z G
Mol Gen Genet. 1982;186(4):572-4. doi: 10.1007/BF00337967.
To investigate the interaction of subunits A and B of DNA gyrase during DNA supercoiling, a Cour mutant of Escherichia coli was obtained and the effect of nalidixic acid on the supercoiling of DNA by wild-type and mutant enzymes was assayed. The enzyme of the Cour strain proved to be more sensitive to nalidixic acid than the wild-type DNA gyrase. Hence the mutation affecting the B subunit can also change the properties of the A subunit, which fact suggests that the two subunits of DNA gyrase are in contact during DNA supercoiling.
为了研究DNA回旋酶亚基A和B在DNA超螺旋过程中的相互作用，获得了大肠杆菌的一个Cour突变体，并测定了萘啶酸对野生型和突变型酶催化DNA超螺旋的影响。结果表明，Cour菌株的酶比野生型DNA回旋酶对萘啶酸更敏感。因此，影响B亚基的突变也会改变A亚基的性质，这一事实表明，DNA回旋酶的两个亚基在DNA超螺旋过程中相互接触。
