Olajos E J, Shopp G, Rosenblum I
Neurotoxicology. 1982 Jul;3(1):146-50.
The effect of various organophosphorus (OP) compounds on 2', 3'-cyclic nucleotide 3'-phosphohydrolase (CNP) activity and the relationship to neurotoxic response was studied. Adult White Leghorn hens were dosed with either diisopropyl fluorophosphate (DFP), tri-o-cresyl phosphate (TOCP), leptophos, or paraoxon. CNP activity was determined utilizing crude homogenates or purified myelin preparations from whole brain, spinal cord, and sciatic nerves at maximal neurologic dysfunction, evaluated as locomotor impairment. Enzyme assays of CNP revealed a decrease in brain CNP activity at the time of maximal locomotor impairment after a single, oral dose of leptophos. Decreased CNP activity was also noted in spinal cord preparations from DFP-treated hens at the time of maximal locomotor impairment. Sciatic nerve CNP activity was not altered following treatment with OP-compounds. Paraoxon, a non-neurotoxic OP-compound, had little effect on neural tissue CNP activity. Neurotoxic OP-compounds, that cause secondary degeneration of myelin (Wallerian), may be associated with decreased brain and spinal cord CNP activity at the time of maximal locomotor impairment.
研究了各种有机磷（OP）化合物对2'，3'-环核苷酸3'-磷酸水解酶（CNP）活性的影响及其与神经毒性反应的关系。给成年白来航鸡分别投喂氟磷酸二异丙酯（DFP）、磷酸三邻甲苯酯（TOCP）、溴苯磷或对氧磷。在最大神经功能障碍（以运动功能受损评估）时，利用全脑、脊髓和坐骨神经的粗匀浆或纯化髓磷脂制剂测定CNP活性。CNP酶分析显示，单次口服溴苯磷后，在最大运动功能受损时脑CNP活性降低。在最大运动功能受损时，DFP处理的母鸡脊髓制剂中也观察到CNP活性降低。用OP化合物处理后，坐骨神经CNP活性未改变。对氧磷是一种非神经毒性的OP化合物，对神经组织CNP活性影响很小。导致髓鞘继发性变性（华勒氏变性）的神经毒性OP化合物，可能与最大运动功能受损时脑和脊髓CNP活性降低有关。
