Oku N, Inoue K, Nojima S, Sekiya T, Nozawa Y
Biochim Biophys Acta. 1982 Sep 24;691(1):91-6. doi: 10.1016/0005-2736(82)90217-6.
The interaction of liposomes containing glycophorin, a major sialoglycoprotein of human erythrocytes, with Sendai virus was studied by freeze-fractures and negative staining electron-microscopy. Viral envelopes were absorbed on liposomal membranes at 0 degrees C. When the temperature was shifted up to 37 degrees C, the viral envelopes fused with the liposomal membranes (envelope fusion). Particles representing viral membrane components formed clusters on liposomal membranes after incubation for more than 1 h at 37 degrees C.
通过冷冻断裂和负染色电子显微镜研究了含有血型糖蛋白(人类红细胞的一种主要唾液酸糖蛋白)的脂质体与仙台病毒的相互作用。在0℃时,病毒包膜吸附在脂质体膜上。当温度升至37℃时,病毒包膜与脂质体膜融合(包膜融合)。在37℃孵育1小时以上后,代表病毒膜成分的颗粒在脂质体膜上形成簇。