Lewis S, Rosenberg N, Alt F, Baltimore D
Cell. 1982 Oct;30(3):807-16. doi: 10.1016/0092-8674(82)90285-9.
A cell line transformed by Abelson murine leukemia virus, called PD, is capable of carrying out kappa-gene rearrangement while growing in culture. Subclones of PD have diverse kappa-gene structures, and some derivatives show evidence of continued joining activity after as many as three subclonings. Analysis of PD sublineages has shown that a rearranged chromosome can undergo secondary kappa-gene rearrangements, producing either a new rearrangement or a deletion of C kappa. Although the PD line actively rearranges its kappa genes, its rearranged heavy-chain genes show little variation, and there is no rearrangement of lambda genes. In PD subclones, DNA fragments representing the reciprocal product of kappa-gene rearrangement are often evident, and they may undergo either further rearrangement or deletion. The implications of multiple rearrangements on a single chromosome and of the maintenance of reciprocal fragments are considered in the context of a model that postulates that the V kappa and J kappa segments are not all organized in the DNA in the same transcriptional direction, leading to inversions rather than deletions during joining.
一种由阿贝尔森鼠白血病病毒转化的细胞系，称为PD，在培养过程中能够进行κ基因重排。PD的亚克隆具有多样的κ基因结构，一些衍生物在多达三次亚克隆后仍显示出持续连接活性的证据。对PD亚系的分析表明，一条重排的染色体可经历二次κ基因重排，产生新的重排或Cκ的缺失。尽管PD细胞系积极重排其κ基因，但其重排的重链基因变化很小，且λ基因无重排。在PD亚克隆中，代表κ基因重排相互产物的DNA片段常常很明显，它们可能进一步重排或缺失。在一个假设模型的背景下考虑了单条染色体上多次重排以及相互片段维持的意义，该模型假定Vκ和Jκ片段在DNA中并非都以相同的转录方向排列，导致连接过程中发生倒位而非缺失。
