Müller B, Reth M
Institute for Genetics, University of Cologne, Federal Republic of Germany.
J Exp Med. 1988 Dec 1;168(6):2131-7. doi: 10.1084/jem.168.6.2131.
Derivatives of the mu-producing Abelson line P8 have been analyzed for L chain gene rearrangements. Two of seven clones studied assembled their V lambda genes while growing in culture. V lambda gene rearrangements occurred only in those Abelson subclones that either were rearranging or had rearranged their recombining sequence (RS) element on both Ig kappa alleles. Our data suggest that (a) RS rearrangements are preferentially initiated in kappa- pre-B cells; and (b) the deletion or inactivation of sequences lying between J kappa and RS is a requirement for the activation of the Ig lambda locus.
已对产生μ链的阿贝尔森系P8的衍生物进行了轻链基因重排分析。在研究的七个克隆中，有两个在培养过程中组装了它们的Vλ基因。Vλ基因重排仅发生在那些在两个Igκ等位基因上正在重排或已经重排其重组序列（RS）元件的阿贝尔森亚克隆中。我们的数据表明：（a）RS重排在κ前B细胞中优先启动；（b）Jκ和RS之间序列的缺失或失活是Igλ基因座激活的必要条件。
