Fernandez-Pardal J, Saavedra J M
Hypertension. 1982 Nov-Dec;4(6):821-6. doi: 10.1161/01.hyp.4.6.821.
Steady state levels of catecholamines (dopamine, norepinephrine, and epinephrine) were measured by the use of radioenzymatic techniques in discrete areas of the kidney (outer and inner cortex, outer and inner medulla) dissected by a "punch" technique from frozen kidney sections of salt-sensitive (DS) and salt-resistant (DR) Dahl rats fed a low or high salt diet. All three catecholamines were present in all areas of the kidney examined. There were gradients of concentrations of each catecholamine in different kidney areas. Renal medullary areas contained proportionally more dopamine than cortical areas. The proportion of epinephrine with respect to the total catecholamine content was relatively high in the inner medulla. Genetic factors and the amount of dietary salt influenced the catecholamine content in specific kidney areas, and these changes were different according to the area considered. DS rats when fed a high salt diet presented increased systolic blood pressure but no increased levels of dopamine in the inner medulla nor of norepinephrine in the outer medulla and outer cortex. Results suggest that either the uptake, release, storage, synthesis, or catabolism of kidney catecholamines is altered in Dahl salt-sensitive (DS) hypertensive rats and suggest specific roles for each catecholamine in discrete areas of the kidney.
采用放射性酶技术，对通过“打孔”技术从喂食低或高盐饮食的盐敏感（DS）和盐抵抗（DR）Dahl大鼠的冷冻肾脏切片中分离出的肾脏离散区域（外皮质和内皮质、外髓质和内髓质）中的儿茶酚胺（多巴胺、去甲肾上腺素和肾上腺素）的稳态水平进行了测量。在所检查的肾脏所有区域中均存在这三种儿茶酚胺。在不同的肾脏区域，每种儿茶酚胺的浓度存在梯度。肾髓质区域含有的多巴胺比例比皮质区域相对更高。在内髓质中，肾上腺素相对于总儿茶酚胺含量的比例较高。遗传因素和饮食盐含量影响特定肾脏区域中的儿茶酚胺含量，并且根据所考虑的区域不同，这些变化也有所不同。喂食高盐饮食的DS大鼠收缩压升高，但内髓质中的多巴胺水平以及外髓质和外皮质中的去甲肾上腺素水平均未升高。结果表明，在Dahl盐敏感（DS）高血压大鼠中，肾脏儿茶酚胺的摄取、释放、储存、合成或分解代谢发生了改变，并表明每种儿茶酚胺在肾脏离散区域中具有特定作用。
