Carter K, Palmer G
J Biol Chem. 1982 Nov 25;257(22):13507-14.
High and low spin complexes of ferric and ferrous heme a have been prepared and characterized spectroscopically. Bis(1-methylimidazole) heme a provides a good model for cytochrome a in both oxidation states while several spectral properties of cytochrome a3 can be reproduced by 1,2-dimethylimidazole heme a3. The visible absorbance spectra of these analogs account well for the absorbance spectra of oxidized and reduced cytochrome oxidase and support the conclusion (Vanneste, W. (1966) Biochemistry 5, 838-848) that cytochrome a provides the major contribution to the spectral changes in the 600 nm band upon reduction. The 655 nm band present in cytochrome oxidase appears to be a characteristic of high spin heme a+3.
已制备出高铁血红素a和亚铁血红素a的高自旋和低自旋配合物，并通过光谱进行了表征。双（1-甲基咪唑）血红素a在两种氧化态下都为细胞色素a提供了良好的模型，而细胞色素a3的几种光谱性质可以由1,2-二甲基咪唑血红素a3再现。这些类似物的可见吸收光谱很好地解释了氧化型和还原型细胞色素氧化酶的吸收光谱，并支持了细胞色素a在还原时对600nm波段光谱变化起主要作用的结论（万内斯特，W.（1966年）《生物化学》5，838-848）。细胞色素氧化酶中存在的655nm波段似乎是高自旋血红素a+3的一个特征。
