Goodman M G, Weigle W O
J Immunol. 1982 Dec;129(6):2715-7.
The metabolic fates of 8-bromoguanosine (8BrGuo) and 8-bromoguanosine-3'5'-cyclic monophosphate (8Br-cGMP) were examined in cultures of murine B lymphocytes. These compounds exert striking immunostimulatory effects upon bone marrow-derived lymphoid cells in vitro. Both 8BrGuo and 8Br-cGMP were resistant to metabolic processing by these cells. That purine metabolic pathways are intact and operant in B cells was demonstrated by the ready degradation and phosphorylation of native guanosine and cyclic GMP. Inaccessibilty of the substrate to the relevant enzymes was ruled out as an explanation by the observation that the brominated compounds also were resistant to processing in broken cell preparations. Moreover, 8BrGuo did not interfere with the cellular machinery for metabolizing native guanosine. The implications of these observations for studying the actions of purine nucleotides, cyclic nucleotides, and their enzymatic processing in B cells are discussed.
在小鼠B淋巴细胞培养物中研究了8-溴鸟苷（8BrGuo）和8-溴鸟苷-3',5'-环一磷酸（8Br-cGMP）的代谢命运。这些化合物在体外对骨髓衍生的淋巴细胞具有显著的免疫刺激作用。8BrGuo和8Br-cGMP都对这些细胞的代谢过程具有抗性。天然鸟苷和环鸟苷酸的快速降解和磷酸化证明了嘌呤代谢途径在B细胞中是完整且起作用的。溴化化合物在破碎细胞制剂中也对代谢具有抗性，这一观察结果排除了底物无法被相关酶作用的解释。此外，8BrGuo并不干扰细胞代谢天然鸟苷的机制。讨论了这些观察结果对研究B细胞中嘌呤核苷酸、环核苷酸及其酶促过程作用的意义。
