Leibowitz J L, Weiss S R, Paavola E, Bond C W
J Virol. 1982 Sep;43(3):905-13. doi: 10.1128/JVI.43.3.905-913.1982.
The coding assignments of the intracellular murine hepatitis virus-specific subgenomic RNA species and murine hepatitis virion RNA have been investigated by cell-free translation. The six murine hepatitis virus-specific subgenomic RNAs were partially purified by agarose gel electrophoresis and translated in an mRNA-dependent rabbit reticulocyte lysate, and the cell-free translation products were characterized by gel electrophoresis, immunoprecipitation, and tryptic peptide mapping. These studies have shown that RNA 7 codes for the nucleocapsid protein, RNA 6 codes for the E1 protein, RNA 3 codes for the E2 protein, and RNA 2 codes for a 35,000-dalton nonstructural protein. Genomic RNA directs the cell-free synthesis of three structurally related polypeptides of greater than 200,000 in molecular weight.
通过无细胞翻译研究了细胞内鼠肝炎病毒特异性亚基因组RNA种类和鼠肝炎病毒粒子RNA的编码分配。六种鼠肝炎病毒特异性亚基因组RNA通过琼脂糖凝胶电泳进行部分纯化，并在依赖mRNA的兔网织红细胞裂解物中进行翻译，无细胞翻译产物通过凝胶电泳、免疫沉淀和胰蛋白酶肽图谱进行表征。这些研究表明，RNA 7编码核衣壳蛋白，RNA 6编码E1蛋白，RNA 3编码E2蛋白，RNA 2编码一种35,000道尔顿的非结构蛋白。基因组RNA指导无细胞合成三种分子量大于200,000的结构相关多肽。
