Koyama K, Otowa T, Yamauchi H, Okabe K, Sato T
Tohoku J Exp Med. 1982 Sep;138(1):17-25. doi: 10.1620/tjem.138.17.
Mitochondrial respiratory function of the liver is disturbed in biliary obstruction, especially in that caused by tumours in the hepatobiliary system. This study aimed to clarify whether a glucocorticoid, prednisolone succinate, is effective to improve the reduced mitochondrial function of the rat liver in obstructive jaundice. Five doses of 5 mg/kg or 25 mg/kg prednisolone succinate were administered at 5 consecutive days to the rats after 1, 3 or 6 weeks of biliary obstruction and to the rats without obstruction, and the hepatic mitochondrial function and contents of cytochromes of these rats were investigated. With 25 mg/kg prednisolone, hepatic mitochondrial function was improved in rats with biliary obstruction of 3- or 6-weeks duration as compared with the prednisolone-untreated group. Compensatory increase or recuperation of the once decreased cytochrome A(+a3) and/or turnover number of phosphorylation was also observed in 25 mg/kg group. Hepatic mitochondrial function of obstruction-free animals was rather impaired by treatment with 25 mg/kg prednisolone. In 5 mg/kg group, above mentioned effects were scarcely observed. It was concluded that the hepatic mitochondrial function in animals with prolonged biliary obstruction, as same as in animals with short-term obstruction, can be improved by the administration of adequately large amounts of prednisolone.
在胆道梗阻，尤其是由肝胆系统肿瘤引起的胆道梗阻中，肝脏的线粒体呼吸功能会受到干扰。本研究旨在阐明糖皮质激素琥珀酸泼尼松龙是否能有效改善梗阻性黄疸大鼠肝脏线粒体功能的降低。在胆道梗阻1、3或6周后，对大鼠连续5天给予5mg/kg或25mg/kg的琥珀酸泼尼松龙，同时也对未梗阻的大鼠给药，并研究这些大鼠的肝线粒体功能和细胞色素含量。与未用泼尼松龙治疗的组相比，给予25mg/kg泼尼松龙后，3周或6周胆道梗阻大鼠的肝线粒体功能得到改善。在25mg/kg组中，还观察到曾经降低的细胞色素A(+a3)和/或磷酸化周转数的代偿性增加或恢复。给予25mg/kg泼尼松龙治疗会损害无梗阻动物的肝线粒体功能。在5mg/kg组中，几乎未观察到上述效应。得出的结论是，与短期梗阻动物一样，长期胆道梗阻动物的肝线粒体功能可通过给予足够大剂量的泼尼松龙得到改善。
