Sun D C, Leak L V
Anat Rec. 1982 Sep;204(1):69-82. doi: 10.1002/ar.1092040110.
The distribution and density of receptors for concanavalin A (Con A) on the surfaces of cells of intact and isolated popliteal and axillary lymph nodes were investigated in the rabbit. Intact lymph nodes were perfused via the subcapsular (marginal) sinus with either Con A peroxidase or Con A ferritin, fixed with glutaraldehyde, and processed for electron microscopy. Both Con A peroxidase and Con A ferritin were distributed on the plasmalemma of lymphocytes, macrophages, neutrophils, plasma cells, reticular endothelial cells, and the vascular endothelium. Counts of Con A-conjugated ferritin particles indicated that the density of Con A receptors was generally similar for lymphocytes, macrophages, and neutrophils but lower on plasma cells. When lymph node cells were isolated by mechanical methods and exposed to Con A ferritin, the label was homogenously distributed on the cell surfaces of most cells. However, Con A binding was significantly higher on the surface of isolated cells than in the intact node. It is suggested that the increase in density of Con A binding sites on isolated cells may possibly be due to an unmasking of cell surface moieties in which additional Con A receptor sites become available as a result of the isolation procedure. The density of Con A ferritin binding sites was also significantly lower on the surface of isolated plasma cells than the lymphocyte and macrophage, suggesting that the density distribution of cell surface saccharides is different for various lymphoid cells.
对兔完整和分离的腘窝及腋窝淋巴结细胞表面伴刀豆球蛋白A（Con A）受体的分布和密度进行了研究。完整淋巴结经被膜下（边缘）窦灌注Con A过氧化物酶或Con A铁蛋白，用戊二醛固定，然后进行电子显微镜处理。Con A过氧化物酶和Con A铁蛋白均分布于淋巴细胞、巨噬细胞、中性粒细胞、浆细胞、网状内皮细胞及血管内皮的质膜上。对Con A结合的铁蛋白颗粒计数表明，淋巴细胞、巨噬细胞和中性粒细胞的Con A受体密度通常相似，但浆细胞上的密度较低。当通过机械方法分离淋巴结细胞并使其暴露于Con A铁蛋白时，标记物均匀分布于大多数细胞的表面。然而，Con A在分离细胞表面的结合明显高于在完整淋巴结中的结合。提示分离细胞上Con A结合位点密度的增加可能是由于细胞表面部分被暴露，在分离过程中额外的Con A受体位点变得可用。Con A铁蛋白结合位点在分离的浆细胞表面的密度也明显低于淋巴细胞和巨噬细胞，表明不同淋巴细胞的细胞表面糖类密度分布不同。
